What is the problem in attached file?

I have done ORR LSV (attached file) at different RPM (400-2000) in 0.1M KOH electrolyte (PH=13) using RDE. My teacher said that it is wrong due to limiting current density. Kindly give me your...

08 September 2019 1,662 2 View

How to do Particle Size Distribution of very small particles?

I used Image j but I didn't get correct result, is there another way to calculate particle size?

05 June 2019 8,862 3 View

What is the correct way of conversion of ppm to ppb?

Please suggest me a correct method of dilution of solutions to convert ppm to ppb. Let suppose I have 0.05 mg/ml solution, it has 50 ppm and I want to convert into different ppb solutions like 50,...

04 May 2019 9,307 1 View

How I can match simulated and own XRD pattern in Origin?

Hi, I have used American Mineralogist crystal database and downloaded .cif files and then used VESTA software to simulate XRD pattern and also have jcpdf number, I want to match with my own XRD...

04 May 2019 1,468 4 View

How can a beginner learn the analysis of X-ray photoelectron spectroscopy (XPS) by himself?

Is there any literature or trilaterals?

04 May 2019 3,309 6 View

Can anyone provide me JCPDS files?

Hi, everyone, I needed JCPDS files of Pt, Cu, PtCu & Cu3Pt, if anyone have then please share with me, I will be highly thankful. File numbers are as follows: PtCu = JCPDS 48-1549 Pt = JCPDS...

04 May 2019 1,856 4 View

What does it mean pass/fail in BET result?

I did BET for my catalyst (PtCu Nano particles) when I got result, I saw this error (given in attached figure), as BET Surface area is too low, what does "fail'' mean? is it incomplete analysis or...

03 April 2019 7,043 10 View

How can a beginner learn the analysis of XRD Powder method by himself?

Is there any literature or trilaterals?

01 February 2019 4,976 12 View

What is the standard electrode potential of Hg/HgO ?

01 February 2019 4,924 3 View

How to convert a potential collected with reference to Hg/HgO to Reversible Hydrogen Electrode?

I have collected a LSV data by taking Hg/HgO as the reference electrode. But I want to convert this one to RHE. Please suggest me the required conversion equation. 0.1M KOH was taken as the...

01 February 2019 6,730 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View