I am looking at ways to measure volume of water droplets in 1-10 uL volume range, pipetted on a hydrophobic surface using image analysis (or other standard methods). I understand in closed format electrowetting on dielectric image analysis can be applied to images of top view of droplets. Can the same be done with open format (where height of droplet might vary)? Is there any open source image analysis tools for same?
https://upload.wikimedia.org/wikipedia/en/7/70/Ion-mask_water_droplet_material_surface_2.jpg
We use software from First Ten Angstroms to measure droplet geometry using side-view images. As far as open-source, I do not know. There are ImageJ plugins out there to analyze droplet images. Here are a couple I found but haven't tried:
https://imagej.nih.gov/ij/plugins/contact-angle.html
http://bigwww.epfl.ch/demo/dropanalysis/
If you assume a spherical droplet, which is valid as long as gravitational effects are negligible (Bond number), the volume calculation follows directly from things you can measure in Image J such as the droplet height, base diameter, and contact angle.
Dear Harikrishnan Jayamohan,
I recommend you imageJ. I use it to measure the diameter of the microdrops in the capillary tubing and also to measure the angle contact (goniometry) to determine the surface tension.
You can also verify the volume gravimetrically by adding the droplets onto the hydrophobic substrate while placed on a high resolution analytical balance. 1 microliter will be equal to 1 milligram on the balance, assuming a specific gravity of unity.
Dear Harikrishnan,
ImageJ may be tool to serve your purpose as already suggested by Mr. Mahdi.
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