Optimisation of organometallics?

Hello, I'm a beginner to computational chemistry, I'm attempting to optimise [Re(OH2)(CO)3(phen))]+ using gaussian. I have tried to optimise the structure using B3LYP method in gen mode SDD for...

20 December 2017 5,029 8 View

How to optimise Re complexes using gaussian?

Hello, I have to optimise [Re(OH2)(CO)3(phen)]+, I have made the structure on gaussview, I have attempted to optimise the complex with B3LYP method in gen mode (SDD for Re and 6-31g(d,p) for...

13 November 2017 4,636 5 View

Does pyramidins melts completely in a PCR reaction?

29 August 2016 2,868 3 View

What are the methods to fragment genomic bacterial DNA to get ~kb size fragments?

What are the conventional methods to fragment bacterial DNA to get ~1-10 kb size fragments? Is there any review paper or other publications out there if it's a very broad set of methods? Any...

25 February 2016 8,998 2 View

Why my HeLa cells are not adherent after freezing?

05 August 2014 2,754 20 View

What could cause Molt4 leukemia cancer cell line to grow very very slowly?

Does anyone have experience with this cell line? I bought this cell line as a frozen sample, and two months after thawing its growth is very slow. I'm using the medium composition as per ATCC .

19 November 2013 6,074 4 View

Is there a particular reason DPV is preferred over EIS or are there any cons or advantages of one over the other?

I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being...

04 August 2013 739 4 View

How to reduce noise during differential pulse voltammetry? Effect of working electrode area?

I am trying to run DPVs using a GCE/glassy carbon working electrode on Ruthenium in 0.2 M acetate buffer (Pt CE & Ag/Agcl RE). When I try using a lower surface area electrode I see that noise...

06 June 2013 6,409 19 View

Effect of layers of graphene oxide deposited on GCE (or other substrate).

Does the number of layers of deposited graphene oxide thickness determine electrochemical (EC) performance of Glassy Carbon electrode? If you say electrodeposit a thicker layer is that good or bad...

04 June 2013 1,607 8 View

Can someone help me in regards to separating Graphene Oxide by centrifugation?

I am preparing Graphene Oxide/GO using Hummers method. The protocol mentions filtering the GO. But that takes a week and it's hard to get the GO flakes off the filter paper. Can we do it faster by...

04 June 2013 1,104 19 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View