Harikrishnan Jayamohan

14 Questions 47 Answers 0 Followers

Questions related from Harikrishnan Jayamohan

Can we mix T cells with stock isotonic Percoll for separation of debris?

I am trying to figure out a protocol to separate cell debris from Jurkat cells using Percoll density centrifugation. Most protocols mention layering cells in PBS on top of stock isotonic Percoll...

12 October 2021 4,149 0 View

What are the critical parameters for femtosecond laser assisted etching of quartz slides?

I understand at high pulse energies you can machine visible patterns on quartz. How do we tune the energy/other parameters to form patterns which are only visible after etching with HF or KOH?

19 January 2021 5,958 1 View

What are the methods to measure volume of microliter/sub-microliter droplet using image analysis?

I am looking at ways to measure volume of water droplets in 1-10 uL volume range, pipetted on a hydrophobic surface using image analysis (or other standard methods). I understand in closed format...

16 November 2016 2,764 5 View

What are the methods to fragment genomic bacterial DNA to get ~kb size fragments?

What are the conventional methods to fragment bacterial DNA to get ~1-10 kb size fragments? Is there any review paper or other publications out there if it's a very broad set of methods? Any...

26 February 2016 9,174 2 View

Does anybody know how to fix this issue with bonding semi-cured PDMS layers (1:5 & 1:20)?

I am trying to bond two layers of PDMS (1:5 & 1:20-binder:PDMS base mix) to make Multilayer soft lithography based device. I am following the protocol used by Stanford Microfluidics foundry...

08 December 2015 4,648 16 View

How do I create a GDSII file from DWG file containing regions (not polylines)?

I have a drawing in dwg format that contains regions and not polylines. I am trying to convert it to GDSII format using LinkCAD. But since the drawing contains regions it somehow ignores certain...

28 August 2015 3,184 2 View

What Materials/Methods are used to prevent fouling in microfluidic devices (involving cells, proteins, nucleic acids)?

Is there a set of materials (glass, plastics etc.) that are usually used for commercial analytical devices involving nucleic acids, cells etc. Like for commercial NGS platforms what materials are...

03 August 2015 8,922 4 View

Is there a standard min cut-off size of E. coli colony for plate counting?

I am trying to plate UV light inactivated E. coli on agar plates to determine CFU count. When I plate the samples (see attached) I see different size of colonies. From

20 April 2015 7,331 9 View

Which is most widely accepted & reproducable DNA electrochemical detection protocols in literature?

Is there any standard protocols which is reproducible for detection of DNA on any form of electrodes. I see tons of papers on electrochemical detection of DNA but does someone have any experience...

17 February 2015 1,030 4 View

What settings to use for DPV hybridization detection in Acetate buffer?

I am trying to run DPV (differential pulse voltammetry) for detection of DNA hybridization in acetate buffer. I have been looking at published work and most of them mention settings used (other...

28 May 2014 8,534 1 View

Is there a particular reason DPV is preferred over EIS or are there any cons or advantages of one over the other?

I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being...

05 August 2013 923 4 View

How to reduce noise during differential pulse voltammetry? Effect of working electrode area?

I am trying to run DPVs using a GCE/glassy carbon working electrode on Ruthenium in 0.2 M acetate buffer (Pt CE & Ag/Agcl RE). When I try using a lower surface area electrode I see that noise...

07 June 2013 6,565 19 View

Can someone help me in regards to separating Graphene Oxide by centrifugation?

I am preparing Graphene Oxide/GO using Hummers method. The protocol mentions filtering the GO. But that takes a week and it's hard to get the GO flakes off the filter paper. Can we do it faster by...

05 June 2013 1,249 19 View

Effect of layers of graphene oxide deposited on GCE (or other substrate).

Does the number of layers of deposited graphene oxide thickness determine electrochemical (EC) performance of Glassy Carbon electrode? If you say electrodeposit a thicker layer is that good or bad...

05 June 2013 1,760 8 View