Harikrishnan Jayamohan

10 Questions 34 Answers 0 Followers

Questions related from Harikrishnan Jayamohan

Can we mix T cells with stock isotonic Percoll for separation of debris?

I am trying to figure out a protocol to separate cell debris from Jurkat cells using Percoll density centrifugation. Most protocols mention layering cells in PBS on top of stock isotonic Percoll...

12 October 2021 4,094 0 View

What are the critical parameters for femtosecond laser assisted etching of quartz slides?

I understand at high pulse energies you can machine visible patterns on quartz. How do we tune the energy/other parameters to form patterns which are only visible after etching with HF or KOH?

19 January 2021 5,902 1 View

What are the methods to fragment genomic bacterial DNA to get ~kb size fragments?

What are the conventional methods to fragment bacterial DNA to get ~1-10 kb size fragments? Is there any review paper or other publications out there if it's a very broad set of methods? Any...

26 February 2016 9,106 2 View

Does anybody know how to fix this issue with bonding semi-cured PDMS layers (1:5 & 1:20)?

I am trying to bond two layers of PDMS (1:5 & 1:20-binder:PDMS base mix) to make Multilayer soft lithography based device. I am following the protocol used by Stanford Microfluidics foundry...

08 December 2015 4,612 16 View

How do I create a GDSII file from DWG file containing regions (not polylines)?

I have a drawing in dwg format that contains regions and not polylines. I am trying to convert it to GDSII format using LinkCAD. But since the drawing contains regions it somehow ignores certain...

28 August 2015 3,135 2 View

What settings to use for DPV hybridization detection in Acetate buffer?

I am trying to run DPV (differential pulse voltammetry) for detection of DNA hybridization in acetate buffer. I have been looking at published work and most of them mention settings used (other...

28 May 2014 8,495 1 View

Is there a particular reason DPV is preferred over EIS or are there any cons or advantages of one over the other?

I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being...

05 August 2013 854 4 View

How to reduce noise during differential pulse voltammetry? Effect of working electrode area?

I am trying to run DPVs using a GCE/glassy carbon working electrode on Ruthenium in 0.2 M acetate buffer (Pt CE & Ag/Agcl RE). When I try using a lower surface area electrode I see that noise...

07 June 2013 6,484 19 View

Can someone help me in regards to separating Graphene Oxide by centrifugation?

I am preparing Graphene Oxide/GO using Hummers method. The protocol mentions filtering the GO. But that takes a week and it's hard to get the GO flakes off the filter paper. Can we do it faster by...

05 June 2013 1,180 19 View

Effect of layers of graphene oxide deposited on GCE (or other substrate).

Does the number of layers of deposited graphene oxide thickness determine electrochemical (EC) performance of Glassy Carbon electrode? If you say electrodeposit a thicker layer is that good or bad...

05 June 2013 1,677 8 View