I do not wish to use the time-consuming cloning/sequencing methods or costly 454 GS platform. The Ion Torrent PGM technology seems to offer modest set-up and running costs. What are your experiences?
Sequencing by Sanger all 16S RNA. You can use universal primers and you will have a good overview of the bacterial community.
La politique de financement est en soit une politique publique. Comment les évaluer dans un travail de recherche scientifique ?
22 July 2024 6,495 2 View
Hi all, I am looking for some syntethised samples of mixed-layer clays to perform infrared reflectance spectroscopy measurements in the VNIR-SWIR-MWIR-LWIR regions. Best regards, Lionel Fonteneau
01 July 2024 3,875 2 View
Please help me to know how an elasticaly mounted circular cylinder for VIV model can be used in Civil Engineering.
12 December 2023 2,497 3 View
Co-expression of multiple proteins in E. coli requires the co-transformation of several plasmids. What is the best protocol for the preparation of ultra-competent E. coli BL21?
29 June 2023 495 3 View
Please, I want some article which talk about the RHA effect on geopolymer concrete properties.
11 June 2023 7,460 6 View
Hello, I am doing PCR on fungal DNA samples and use EF1 and EF2 primers and the New England Bio Labs Phusion Taq Polymerase. I have had successful PCR reactions but over the past month I have been...
23 June 2021 1,391 4 View
I was reading the T7 RNA polymerase protocol and noticed the following sentence regarding incubation time: "Incubate at 37°C for 1 hour. For shorter (< 300 nt) transcripts incubate at 37°C for...
17 June 2020 1,918 0 View
Extraction of viral RNA from cell supernatants or from plasma/serum is supposed to be possible using TRIzol or TRIsure, provided that glycogen is added. What is your own experience? I want to...
23 March 2020 7,881 7 View
Hello, Has anyone used the Roche HighPure viral RNA kit to extract RNA from cells? The idea for me is to use the same kit to extract RNA from supernatants of infected cells and from the cells...
23 March 2020 3,939 3 View
I am planning to run a glucose uptake experiment in mice (In vivo). I would like to inject H3-2DG and just measure the percent of H3 in different tissues. No clamp experiment, but I was thinkMing...
18 February 2020 9,669 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View