Hello need help on Microtome. Anyone experiencing the blade cutting deeply FFPE tissue block after a few sections of the same tissue?

The blade is set at a clearance angle of 5 degrees at which it has been sectioning very well. In the past few days with no change of blade type or type of wax. The blade has been cutting in too...

20 October 2020 769 5 View

Gromacs Protein com Ligand com distance, which is the right selection syntax for gmx distance?

I would need to check the distance between the two centers of mass of a Protein and of an organic Ligand (the resname and the resnumber of the ligand are both known), inside a system containing...

22 April 2020 3,696 4 View

How to isolate keratinocytes from fibroblasts in a co-culture using medium?

Currently I'm culturing primary human fibroblasts and keratinocytes from a skin punch in medium (DMEM with 20% FBS and 1% P/S). I would like to culture these cells separately, but I was wondering...

01 March 2020 9,425 3 View

Alternative for Qvotek microinjection needles?

A while ago the website of Qvotek went offline, and their supplies haven't been open for ordering. We were using their custom 5 micron micropipettes/needles/tips for microinjections in zebra fish...

11 November 2019 2,566 3 View

Measuring leadership Effectiveness?

I am looking for an existing tool that would assist in measuring sustainable practices on leadership effectiveness .

18 August 2018 3,640 4 View

What is the potential for individual giving programs for organizational sustainability in Africa? studies?

I am interested in finding out studies conducted or papers presented on individual giving in Africa. Please share. Best regards

13 August 2018 2,971 1 View

What would be the most optimal medium for primary human VSMC culturing?

As stated in the question, I would like to know what kind of culturing medium is generally used for culturing primary human VSMC. Currently for murine cells I'm using DMEM with 10% FBS, Pen/Strep,...

10 April 2017 7,787 4 View

Thoughts about professional certification such as CISSP, CSSLP, CISA, CISM, etc. as job requirement for professors.

Do you feel that computer science and information systems programs should also require professors to have professional certifications in addition to the normal publishing requirements for tenure...

06 October 2013 1,633 6 View

Publishing in different journals vs Publishing in similar journals

For the same number of papers, is it better to publish in different journals (averaged fewer papers per journal) or publish in a few journals (averaged more papers per journal)? Does anyone have...

27 August 2012 1,223 72 View

Please can anyone refer me to a site that can give me a step-by-step procedure for artificial neural network analysis in Matlab?

01 January 1970 2,939 9 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Share a copy of RaFD Dataset?

I tried to get this dataset from the original link but no one approved my requests for weeks. Can someone share with me a private link to use? You can send it to my institutional email:...

14 February 2021 1,908 1 View

Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell.?

Various schools have emerged in the study of comparative literature. Mention the most prominent of them in a nutshell. And he talked about one of them?

09 February 2021 1,099 8 View

Science Teacher's Attitudes Towards Career Learning Index among Senior School Students in Chemistry?

Full discussion of this project topic

09 February 2021 2,534 3 View

Where can I find the Affiliate Stigma Scale developed by Mak and Cheung?

Need the scale for a dissertation. Would be better if I could get it for free since my work is not funded. I need the English version of this scale.

09 February 2021 4,022 3 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Hi, Good day! Do you have related studies about my title, "Factors Affecting the Senior High School Students' Stress Tolerance in Problem Solving"?

I am new researcher. I want to learn more and I need your help. Thank you.

04 February 2021 6,834 4 View