What is the unit of light pollution ?

How light can be polluted

09 October 2019 2,867 5 View

What are the problems tissue engineered dental enamel and dentin could not be created in desired shape and size ?

This could prove to be a good alternative treatment for dental caries

09 October 2019 8,947 2 View

Which are the second line treatment drugs shifted to first line of treatment of epilepsy in human?

What are the new drugs supposed to treat epilepsy

08 September 2019 2,951 5 View

How to identify the sources of enzymes in analytical chemistry of biological processes in human?

Presence and quantification of enzymes is possible but source determining is difficult as source may be microbial/ viral/ healthy or diseased tissue

07 August 2019 1,813 4 View

What are the effects of extreme fluorosis on health/ histological pictures of dental pulp in human teeth?

Fluorosis is affecting osseous tissue and may result in crippling situation of person with difficulties in walking

07 August 2019 9,676 3 View

How much is the atmospheric pressure in sky distant from the planets and satellite?

Has anyone ever tried to see this pressure

06 July 2019 3,339 3 View

In which spectrum of electromagnetic radiation dark energy belongs?

Dark energy may be towards transparency and difficult to see

06 July 2019 1,294 2 View

How much is the change in size of molecules in gaseous state under extreme pressure and vacuum?

Under pressure and vacuum molecular motions are known but size is unknown as far as I am having knowledge

06 July 2019 3,794 3 View

The temperature difference between malignant lesion of internal organ and surrounding healthy tissue and malignant and benign tumours in human ?

How to measure the temperature during surgery in sterile area and which instrument is suitable for this

03 April 2019 3,219 1 View

The images of sun in day time and noon are real or refracted?

In water wooden stick will show two components of image one without refraction/ real and one refracted What is the status of sun we are seeing

03 April 2019 5,356 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

No title

22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View