Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

How to measure the pH of Peat Soil?

Peat possess organic content above 75% and is highly acidic in nature (i.e. pH < 4). Before commencing stabilization, it is mandatory to know about the pH level of peat. Therefore, your...

18 February 2021 9,424 7 View

How to Measure Consumer Willingness To Change from petroleum-fueled vehicle to electric vehicle?

I wanted to calculate the probability of one consumer shifting from using a petroleum-fueled vehicle to an electric vehicle. Since my background is in engineering I do not know much about how to...

15 February 2021 9,190 5 View

Having trouble mounting the sections on the slides for IHC staining, what solution should I use?

Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...

08 February 2021 6,880 3 View

How to use Image Lab to take an image for the bands and the ladder in the same image merged together in Western Blot?

Hello everyone. I was trying to use Image Lab software to take an image of both the ladder and the samples merged together in one image to see the position of the bands according to the ladder MW,...

04 February 2021 9,996 2 View

Need a research paper for multi speaker and single microphone acoustic echo cancellation?

Hi, I am working on a problem that involves multiple smartphones/tablets. All devices are playing the same sound with synchronization difference up to +- 5ms. Now, there is an echo issue and I...

02 February 2021 5,929 3 View

Can I use the result from a pilot study to decide the minimum sample size ?

Can I use the result from a pilot study to decide the minimum sample size required to compare the means using independent sample t-test ? Or any other way that can help me to determine the...

29 January 2021 7,677 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

How to make a model of trachea (human airway)?

I want to carry out flow analysis of air through tachea (human airway). How can I draw it in a Solidworks after taking a CT Scan image?

14 December 2020 5,031 2 View

Histological scoring of acute inflammation in Trachea?

Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli

05 July 2020 7,932 3 View

Should I always dilute cDNA before PCR amplification?

Please I need help! I have some tracheas samples and viral RNA has been extracted (commercial kits) and cDNA was synthesised from total RNA ~ 1 microgram through two-step with target specific...

05 June 2019 3,295 4 View

How to dissect and image the adult trachea of Drosophila?

Drosophila Trachea is important for understand the epithelial organisation. But there is no current protocol to dissect adult fly.

31 December 2017 1,094 0 View

I am trying to induce lung cancer by intubating cancer cell line in murine lung . So far , I have little success . Can any one suggest me a protocol ?

I am trying to induce l by intraline . So far I have little or no success, most of the mice develop tumor in trachea. Not all the mice develop tumor at same time. Although, I got some tumor in...

30 December 2017 2,165 4 View

I am trying to induce lung cancer by intubating cancer cell line in murine lung . So far , I have little success . Can any one suggest me a protocol ?

I am trying to induce l by intraline . So far I have little or no success, most of the mice develop tumor in trachea. Not all the mice develop tumor at same time. Although, I got some tumor in...

11 December 2017 3,680 4 View

What is standard way to present DMA analysis for publication?

For biomechanics expersts: I have done a DMA frequency sweep test to compare between native trachea cartilage and Tissue engineered decellularised cartilage. ( viscoelastic fingerprint ) if any...

11 December 2017 6,974 0 View

Does anyone have any suggestions for keeping older mice alive during urodynamic studies?

I've adapted a procedure for mice from a collaborator that uses rats for urodynamic studies. I use 0.5g/mL urethane to anesthetize mice. I start off with 1.2g/kg subcutaneous, and increase the...

01 February 2016 8,450 2 View

How can I collect the primary rabbit tracheal epithelial cells ?

31 December 2014 2,084 4 View

Are there any methods to prevent contamination outgrowth in primary Alveolar macrophage cultures isolated by BAL???

Our lab is attempting to study trans-infection of HIV-1 from primary Alveolar macrophages. The Alveolar macs are provided from HIV+ BAL samples. Issues are that the cultures are obviously...

07 August 2014 5,265 4 View