How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

I want to convert Methane gas to liquid Methanol using photocatalysis. Can you please suggest me a good paper which I can study ?

There are a number of publications regarding this, but none of them are clear and show the mechanism of gaseous Methane conversion to liquid Methanol.

06 January 2021 364 3 View

Can wavelet packet sub-bands be stacked together to form an image?

I have a 1D signal and I have done wavelet packet decomposition on it which is giving several sub-bands. Can I stack these sub-bands (one below other) to form a 2D matrix and hence an image...

27 November 2020 1,269 5 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

what are the possible reasons for Crop Water Stress Index values higher than 1 under moisture stress condition?

I have determined CWSI using the theoretical approach of Jackson et al., (1982) based on canopy temperature measurement in chickpea. at the final stages of crop growth some of my values of CWSI...

28 October 2020 9,992 3 View

Do always a Good paper depends on How much Citations it Received. I have seen many Good Research Papers with zero or less Than 10 Citations?

Its a Research Paper related Question

22 October 2020 6,179 4 View

Help in cloning, should I create 5' phosphate group on digested vector?

Hello all, I am trying to introduce an annealed DNA fragment into the double digested vector. The ssDNAs were ordered without a phosphate group modification. The vector is digested with XhoI and...

20 October 2020 8,357 5 View

Can anyone explain the calculation process of integrated biomarker response (IBR) used in chronic toxicity study with an arbitrary dataset?

Video tutorial on IBR calculation or stepwise procedure..

05 October 2020 9,279 3 View

Could someone provide the permeability value for 2% Chromium doped BiFeO3?

Any close value will also work.

03 October 2020 8,067 4 View

Purification of annealed oligonucleotides after annealing procedure?

Hello all, I will do oligonucleotides annealing for the first time which will be later used for cloning without any more modification. Can any of you kindly suggest if I should purify the annealed...

16 September 2020 3,881 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

[Covid-19] GGO Evaluation - CT imaging of the lungs ?

13 January 2021 3,115 4 View

Can I measure lung weight after formalin fixation?

Hi, I have simple quick question. Can I make a measurement of mouse lung to evaluate lung metastasis after formalin fixation? Is it not reliable? Thanks.

11 January 2021 3,178 3 View

How to make a model of trachea (human airway)?

I want to carry out flow analysis of air through tachea (human airway). How can I draw it in a Solidworks after taking a CT Scan image?

14 December 2020 5,031 2 View

Immortalized pulmonary microvascular endothelial cell to buy?

Hello, I would like to purchase an immortalized cell line for pulmonary microvascular endothelial cells (MVECs), but I could not find any. I looked up websites, like ATCC, Lonza, Cedarlane, and...

15 November 2020 8,085 3 View

Should I perform transcardial perfusion by PBS before lung isolation for RNA expression analysis?

Hey, I am planning to dissect rats and isolate Lung tissue samples to be stored in RNA stabilizer for later analysis. - Should I perform trandcardial perfusion by PBS ? - Is it necessary to...

21 September 2020 1,822 5 View

What are the correct boundary conditions for lungs in a breathing machine simulation?

Dear Colleagues, We are trying to simulate flow in a breathing machine and need to put the boundary conditions for lungs. Question: is it possible to set adequate boundary conditions for lungs,...

07 September 2020 8,670 4 View

What is the best software (for biomedical engineers) to design professional illustrations?

I am not a graphic designer! But now, I am looking for a Windows-based software to help me in the easy drawing of sophisticated 3D illustrations in the field of biomedical engineering and...

10 August 2020 5,034 7 View

How can I accelareate dehydration of mouse lung with 10% sucrose for frozen section?

09 August 2020 4,967 1 View

CT Lung Image database for infected patients with COVID-19?

Dear Researchers, Could you please suggest me an available CT Lung Image database for infected patients with COVID-19. Thank you all in advance.

13 July 2020 9,733 6 View