What are the important data, parameters and software needed to predict a model the check the impact of climate change in a particular region?

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Different between gene centric approach and assembly approach in omics study ??

different between gene centric approach and assembly approach in omics study ??

10 November 2018 5,563 0 View

How to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??

how to avoid primer dimer formation ?? im getting primer dimer even after increasing the temperature, increasing DMSO , what should be done ??

01 February 2018 3,327 7 View

How can I access the Phylogenetic diversity of a region using 16s rRNA amplicon sequences?

I have a 16s rRNA amplicon sequences (next generation sequence), I want to find out the phylogenetic diversity of a region, How can I do that? please tell me the different approaches. thanking...

01 January 1970 9,267 2 View

What is the reason for Primer Dimer formation in a PCR reaction? Does PCR reagents have any role in Primer Dimer formation?

While doing the PCR amplification of metagenomic DNA, I got stuck with no visible bands on gel. But there is always the presence of primer dimers in the gel. I had added about 2.5ul of DNA...

01 January 1970 7,031 5 View

Which bioinformatic tool is feasible to explain 16s metagenomic data ?

I have 16s metagenomic raw data to be analysed , please describe me different software and its work application to analyse it with respect to diversity ?

01 January 1970 5,329 3 View

What is the best software to prepare a predictive model on climate change on normal 64 bit laptop ?

I have to prepare a predictive model on effect of climate change on iceland/ frozen soils, please suggest me a tools and how can i achieve this ?

01 January 1970 3,501 3 View

Does anybody know how to extract Plasmid DNA from isolated genomic DNA.

I was working on plasmid isolation from an organism but i didn't got plasmid via plasmid isolation kit. However, later on i extracted the gDNA from that portion of the given organism, luckily i...

01 January 1970 3,181 5 View

What are the various methods to estimate Co2 and Methane emission from soil? what are the equpiments/instruments through we can measure the emission?

hello, i want to estimate carbon dioxide and methane from soil , any suggestion and recommendation will be most kind , thanking in anticipation .

01 January 1970 7,701 4 View

What are the best primer for Methanogen amplification ?

hello everyone , i want to check the diversity of Methanogen of a particular soil , please suggest me any idea or pipeline for this analysis, and also suggest me a particular primer for the...

01 January 1970 5,722 1 View

How do you use gel-extracted DNA (size almost 1500-3000bp) in a new PCR reaction?

My primers amplification got many time primer dimer, and i did not get my desired band in pcr amplification. I want to cut the genomic DNA from the gel, and use it as the template for a new PCR...

01 January 1970 9,775 7 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

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02 March 2021 2,431 3 View

Nagalakshmi Radhakrishnan

weather parameters mainly temperature and rainfall

at least 40 years average temperature and rainfall data need to find climate change. if the region is cold region ice balance also calculate to find the albedo range is easy to find the temperature change

Abhijit Mitra

Temperature and CO2 data are very relevant

Sandeep Vm

Rainfall and temperature are the major parameters shall be considered to study the climate change over a regions. The change in pattern of rainfall intensity and distribution also could be analyzed.

Temp, CO2 level and salinity (if in the coast)

Myron King

As Sandeep Vm say above. Also realize that is the root to understanding the physical or natural impact - don't forget about the societal / human impact including economic, infrastructure, and livelihood aspects.