I would like to know the basis of selecting a housekeeping gene for a real time PCR. Does it vary with the gene that we are studying?Particularly membrane proteins and enzymes.
housekeeping genes are used as control just because it's assumed that their expression do not vary along time and samples, whatever the sample is treated or not. but it's just the theory, some change can be obversed. in the housekeeping you can find ABL1 BCOR CCDC106 CCDC50 CCL5 CDKN1A CDX2 EIF4H GAPDH HDC HNRNPK HRH1 HRH2 HRH3 IGLL1 Inpp4b KCNH2 MAP1A MN1 MS4A3 PSMD6 SF3B2 SLC25A3 SPNS3 TBP UBE2I VPS4A.