How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View

Is it possible to calculate the energy stored (kWh) in a EV battery by knowing the cathode discharge potential and specific capacity properties?

For example, if I have the following data for a LiNi0,8Co0,15Al0,05O2 compound used in the cathode: - Specific capacity = 200 mAh/g - Average voltage = 3,7 V I have no information about the...

16 January 2021 3,038 2 View

PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

Can you suggest a sub-problem and possible objectives regarding to this thesis/research study?

03 November 2020 8,155 3 View

DNA disappears from gel, whats going on?

Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it...

28 October 2020 4,427 4 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

31 January 2021 7,704 4 View

How to extract polysaccharids from Aloe gel?

I 'm trying to extract polysacharides from Aloe leaf and tried ethanol percipitation, but after getting the pellet. I used the protocol from following reference following the steps, but I don't...

27 January 2021 7,049 5 View

How to get structure and topology for glycosaminoglycan (GAG) of arbitrary length?

Hello all, I want to do glycosaminoglycan (GAG) simulation in Gromacs. Initial structure and CHARMM topology for short chains can be obtained from Charmm GUI glycan modeler. But I have to take...

23 December 2020 6,092 2 View

Someone knows of a DNA purification protocol from polysaccharides (for brown seaweed extracts)?

Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...

06 October 2020 1,500 1 View

Can any one explain or send a standard protocol for Ulvan Polysacharide extraction from sea weed/algae ?

One of my student is interested in studying the Prebiotic application of ulvan polysaccharide. She extracted polysaccharide using hot water extraction and ethanol precipitation (Following an...

02 September 2020 3,530 3 View

Polysaccharides that were precipitated with ethanol could not be redissolved with water, why?

Hi, I am working with polysaccharides from plant samples. One of the purification steps is to add ethanol to the water extract to precipitate polysaccharides. However, in some cases, the...

17 March 2020 8,361 3 View

Which rapid in-line/on-line method could be used to ensure extraction yield for instant coffe and how to calibrate it on chemometric methods?

There is a need to ensure extraction yield of the soluble coffee process, thus we need a method to analyze carbohydrate degradation products produced after hydrolisys of polysaccharides due to...

11 March 2020 3,308 2 View

What books on anticancer potential of polysaccharides obtained from fungi recommend me to read?

I am investigating the medicinal potential of the polysaccharides present in edible fungi native to my country and that can help prevent or treat some types of cancer.

16 February 2020 7,775 3 View

What books on anticancer potential of polysaccharides obtained from fungi recommend me to read?

I am investigating the medicinal potential of the polysaccharides present in edible fungi native to my country and that can help prevent or treat some types of cancer.

01 February 2020 2,317 2 View

What is the best way to cleave GAGs (HS/CS) from protein samples for western blotting?

I have Heparanase III and Chondroitinase ABC which I would like to use to cleave the heparan sulfate and chondroitin sulfate chains off their core proteins before I run the samples on a western...

27 January 2020 496 1 View