Could you please help me to have a good idea on "Commercial scale of Plant Tissue culture production" or get reference materials regarding this topic, including;
+ Major aspects should consider
+ Media preparation guideline for small/large scale production
+Media Preparation for research scale level with different treatment and replication levels
+Budget planning
P.S: I've studied all the theories on plant tissue culture, I just want to know how to apply them for a commercial scale of production.
Dear Akila,
Commercial production of tissue culture plants is quite different to academic production. For bussines you must have into account:
- Market
- Technical aspects of the processes and the quality of your final product
- Legal aspects for production and commercialization
You must be clear of the product you want to sell, the market requirements, the quality required and offered, and the availability of protocols in order to produce your plants. Remember, market is far more demanding than the worst academic examiner you can imagine. In addition, economic aspects, in particular non-expected expenses, are one of the main limitations for biofactories development.
Tissue culture laboratories can be a good bussines if you identify a good market, but the economical and technical inputs are high, and not always easy to find in order to respond with a good quality product or in sufficient ammounts.
I advice you to review papers related to tissue culture in oil palm to have an idea of things you must have into account.
A good example in oil palm could be found in:
Wong, G; Tan, CC; Soh, AC; Chong, SP. 1999. Clonal propagation of oil palm through tissue culture. The Planter (Malaysia) 75 (878): 221-230.
Wong, G; Tan, CC; Soh, AC. 1996. Large scale propagation of oil palm clones- Experiences todate. Proceedings Third International Symposium on in vitro culture and horticultural breeding. Acta Horticulture 447: 649-658.
Hopefully, you will find these comments and information useful.
Sincerely yours,
Pedro
I agree with the previous answer, it would greatly depend on the purpose and type pf product you aim to produce.
here is a reference review to help give you an idea about pharmaceutical production in plant tissues/cultures and the issues that are applicable:
Production of Pharmaceutical Proteins in Solanaceae Food Crops
MM Rigano, G De Guzman, AM Walmsley, L Frusciante, A Barone
International journal of molecular sciences 14 (2), 2753-2773
Hi!
As per your PS you have some knowledge in plant tissue culture that too theoretical knowledge but not practical knowledge unless otherwise you have some practical knowledge it is very difficult to manage a plant tissue culture lab.
Pedro mentioned excellent points thoroughly follow them.
It is fine Giorgio mentioned name of the article, what plants you want to culture. May I know at least the name of the plants?
Akila it is not as easy as you read in the article, you need a perfect lab including quite sufficient place to harden your plant-lets including green houses etc. Solanum plants are a bit difficult to handle, I have seen few questions about horticulture plants like brinjal, tomato etc.
I worked with banana in commercial scale, an easy plant to manage provided if you follow the techniques correctly, still I see some questions about that some are still getting problems.
Kindly do not think otherwise, you have simply mentioned and asking how to manage lab. Google it you get nice information as well go for NCBI Pubmed you get very nice information about the plants what you want to culture as well standard procedures available for many plants.
First of all you need an aseptic lab (free of contamination) otherwise contamination oust out everything in days, patience is very much needed to manage right from preparation of media, collection of material, inoculation, producing plant-lets, hardening finally marketing.
First go for pilot lab, if you succeed in that then go for commercial scale.
Wish You Good Luck!!!
Hallo
Excellent answers are already given about the requirements of a plant tissue culture laboratory. Another guideline you can get from this book by Kyte L. 1987. Plants from test tubes: An introduction to micro propagation. Basically it is a good practical book from planning and building the laboratory, managing the lab, cost , marketing, aseptic conditions and laboratory equipment.
I recommend the fourth edition (2014 ??)
http://www.amazon.com/Plants-Test-Tubes-Introduction-Micropropogation/dp/1604692065
There is a Kindle electronic version which is less expensive from Amazon. It is available immediately.
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