How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

How will DeepMind AlphaFold2 solution of the core problem of protein structure prediction from sequence impact biology and biophysics?

AF2 may most strongly impact experimental determination of protein structure by multiple methods with possible benefits and negative impacts over time. Some areas may need to quickly pivot or...

13 December 2020 6,026 5 View

Why is SV40 not considered an oncogenic virus?

Hello, SV40 is not considered an oncogenic virus in this review:Oncogenic viruses and cancer Why is SV40 not considered an oncogenic virus when it produces the large T antigen, which is...

04 December 2020 8,374 5 View

What statistical method to use to identify potential confounders in a large set of explanatory variables?

I am working on identifying biomarkers of the outcome of treatment in a disease. I have 20 potential biomarkers and more than 30 potential confounders. Actually, I know that some of those...

11 November 2020 3,794 3 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

Can anyone explain how to calculate BRI (Biomarker response index)?

Hello everyone, Can anyone tell me how to calculate biomarker response index (BRI)? Suppose I have three treatment sets and one control set of plants. I have considered many cytotoxicity,...

28 October 2020 9,304 3 View

Is it possible to use lyophilized blood plasma for "omics" analysis?

Has anyone used or read about blood plasma lyophilization (my interest would be for safe transport/conservation of samples over long distances) for posterior analysis of metabolomics/...

26 October 2020 7,296 7 View

Converting LipidMAP IDs to HMDB IDs in bulk?

I have a list of LipidMAP IDs for a bunch of metabolites and I need them all converted to Human Metabolite Database IDs. I have over 300 entries so I need a way of converting these IDs in bulk,...

20 October 2020 3,372 4 View

Can anyone explain the calculation process of integrated biomarker response (IBR)? Any specifical software?

Hi. I'm working with soil enzymes and I have an issue because i saw several papers that used "integrated biological response" or IBRv2 to integrate data from enzymes and build a hexagonal star...

11 October 2020 5,131 3 View

Where to find cancer datasets composed by clinical markers and gene expression levels?

Currently, I am validating a new machine learning approach. But, I need to find different cancer datasets composed by clinical markers and gene expressions. For now, a just found MMRF data set...

11 October 2020 9,012 2 View