Can anyone help me with using DLS for Antibody accumulation analysis?

02 March 2016 3,754 5 View

Can anyone give some advice concerning mAb conjugation to Nanoparticles using Maleimide-Thiol chemistry?

Hi All, I am working on conjugating mAbs to the surface of PLGA-PEG-MAL nanoparticles. Free thiols are added to the mAbs using Traut's reagent, which will then be added to the NP suspension. I am...

02 March 2016 2,577 8 View

Would the acidity caused by TCEP*HCl reduction be able to denature/deactivate an antibody?

Hi All, I want to reduce coupled Anti-EGFR mAbs for use in active-targeted nano-constructs. I want to use TCEP*HCl as my reducer but know that it causes (and actually needs) an acidic pH when used...

02 March 2016 4,437 11 View

Around what pH would antibodies tend to lose their activity?

Hi All, I am working with mAbs that are being exposed to a low pH (around 3 at the lowest) and was just wondering if that might be enough to cause them to lose some activity or even denature....

02 March 2016 4,284 4 View

What effects would raising PBS concentration above the usual "1x" dilution have on antibodies in solution?

Hi All, I am considering raising the concentration of my PBS solution to buffer against a pH drop during a mAb reaction step. Would that increase have any effect of the structure or affinity of my...

02 March 2016 6,018 4 View

EDTA's role in keeping antibodies from accumulating in solution?

Hi All, I am seeing protocols suggest including EDTA with a reducing agent when reducing disulfide bonds responsible for antibody accumulation and was just wondering how the EDTA's chelating...

02 March 2016 642 4 View

Why would Nanoparticles coated with anti-EGFR mABs be taken into the cell via RME while anti-EGFR mABs alone inhibit the EGFR without being taken up?

Hi All, I am wondering why Nanoparticles with conjugated mABs on their surface as targeting agents are successfully taken up by cells at the receptor site through Receptor-Mediated Endocytosis but...

31 December 2015 1,612 4 View

What is the effect of Maleimide surface coverage on thiol conjugation efficiency?

31 December 2015 5,572 8 View

Any advice on handling & storing antibodies made for infusion?

08 September 2015 3,277 7 View

How would I induce desorption in antibodies physisorbed to the surface of polymeric nanoparticles without denaturing the protein, would salts work?

06 July 2015 6,079 5 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View