Eduard Fidler

11 Questions 26 Answers 0 Followers

Questions related from Eduard Fidler

Can anyone give some advice concerning mAb conjugation to Nanoparticles using Maleimide-Thiol chemistry?

Hi All, I am working on conjugating mAbs to the surface of PLGA-PEG-MAL nanoparticles. Free thiols are added to the mAbs using Traut's reagent, which will then be added to the NP suspension. I am...

03 March 2016 2,602 8 View

Would the acidity caused by TCEP*HCl reduction be able to denature/deactivate an antibody?

Hi All, I want to reduce coupled Anti-EGFR mAbs for use in active-targeted nano-constructs. I want to use TCEP*HCl as my reducer but know that it causes (and actually needs) an acidic pH when used...

03 March 2016 4,465 11 View

What effects would raising PBS concentration above the usual "1x" dilution have on antibodies in solution?

Hi All, I am considering raising the concentration of my PBS solution to buffer against a pH drop during a mAb reaction step. Would that increase have any effect of the structure or affinity of my...

03 March 2016 6,046 4 View

Around what pH would antibodies tend to lose their activity?

Hi All, I am working with mAbs that are being exposed to a low pH (around 3 at the lowest) and was just wondering if that might be enough to cause them to lose some activity or even denature....

03 March 2016 4,314 4 View

EDTA's role in keeping antibodies from accumulating in solution?

Hi All, I am seeing protocols suggest including EDTA with a reducing agent when reducing disulfide bonds responsible for antibody accumulation and was just wondering how the EDTA's chelating...

03 March 2016 671 4 View

Can anyone help me with using DLS for Antibody accumulation analysis?

Hi All, I am trying to use DLS to verify that I am successfully reducing disulfide bonds responsible for the accumulation of mAbs in solution, yielding single uncoupled antibodies. Has anybody...

03 March 2016 3,812 5 View

What are the effects of polymer size/MW on Nanoparticle formation?

Hi All, I am wondering how using polymers of differing molecular weights would affect the formation/properties of nanoparticles and why these differences would come about. Thank you! EF

02 February 2016 9,939 7 View

Why would Nanoparticles coated with anti-EGFR mABs be taken into the cell via RME while anti-EGFR mABs alone inhibit the EGFR without being taken up?

Hi All, I am wondering why Nanoparticles with conjugated mABs on their surface as targeting agents are successfully taken up by cells at the receptor site through Receptor-Mediated Endocytosis but...

01 January 2016 1,641 4 View

What is the effect of Maleimide surface coverage on thiol conjugation efficiency?

Hi All, I am working on a conjugation of a peptide to PLGA-PEG-MAL nanoparticles. I am currently making nanoparticles with PEG strands having 20% MAL endcaps, 80% methoxy. In theory, raising the...

01 January 2016 5,603 8 View

Any advice on handling & storing antibodies made for infusion?

Hi All, I am working with the Erbitux (Cetuximab) monoclonal antibody and intend to conjugate it to PLGA-PEG nanoparticles for targeting, using maleimide. I have Erbitux from BMS that is ready...

09 September 2015 3,308 7 View

How would I induce desorption in antibodies physisorbed to the surface of polymeric nanoparticles without denaturing the protein, would salts work?

Hello All, I am trying to release antibodies (C225/Cetuximab/Erbitux) that have been physisorbed to the surface of PLGA-PEG-MAL nanoparticles without causing them to denature irreversibly. I am...

07 July 2015 6,097 5 View