Why calcium oxide nanoparticles becomes black at 650 degree temp?

Please tell me, which temp can I select for neglect black color of the Calcium oxide nanoparticles ..

14 January 2021 5,341 5 View

Why Porphyrin is purple in color?

Porphyrin solid is purple in color, what factors are responsible for purple color of the porphyrin?

29 November 2020 199 2 View

After successful docking, how you can decide whether ligand act as an activator or inhibitor?

The interaction between drug target and ligand (drug) was performed with the help of biotools or online server(autodock or swissdock). After successful docking, how you can predict desired...

14 February 2020 4,434 15 View

Cosine Similarity between columns of two dataframes of differing lengths ?

Hi All I have text column in df1 and text column in df2. The length of df2 will be always > length of df1. I want to calculare cosine similarity for every entry in df1[text] against every entry...

30 December 2019 7,453 1 View

How to determine if a white colored precipitate on the surface of an extruded aluminium sample (multi-port tube) is aluminium hydroxide?

Dear researchers, I have some multiport extruded tubes with a certain white precipitate visible on the iner side of the tubes and would like to know a way to determine what that precipiate...

10 November 2019 3,318 7 View

How to measure FWHM from XRD analysis?

From XRD analysis how to measure Full Width Half Maxima (FWHM)?

15 September 2019 4,589 5 View

Is there any way we can prove or support the consequences the proton entering the earth's atmosphere during geomagnetic condition have on human being?

During SEP event, many protons of different energy level enters the earth's atmosphere. In the history there has been several such circumstances like 12 hr power cut in Norway during 20th...

26 June 2019 7,932 2 View

If we become able to replicate the Connectome into a machine, like robot, will that robot have the power of AI and emotions?

Future possible use of connectome in machine learning. How far can we go?

03 May 2019 2,039 8 View

Which Nano-particles have more heat carrying capacity?

Which Nano-materials have high fire point, flash point, thermal conductivity and heat transfer coefficient. So that it can be used in a coolant incorporated with base fluids.

18 March 2019 8,869 4 View

What would be the reason behind formation of the W/O emulsion instead of O/W emulsions at higher concentration of material dissolved in oil?

I am trying to make the Oil in Water emulsion. If I dissolve 1% my material in oil then it forms O/W emulsions. But when I use concentration of my material Above 5 % then it forms W/O...

23 January 2019 4,396 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View