How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

Can zinc based fixatives be used after enzymatic dissociation of neural tissue?

I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can...

21 January 2021 4,430 1 View

Is there a technique to store fixed cells after flow for later RNA analysis?

I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...

21 January 2021 9,722 3 View

Test for silicone residue from silicone part?

I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...

09 December 2019 4,246 3 View

ANSYS Meshing Issue - How To Mesh Complicated Geometry (~80,000 Faces)?

I am attempting to mesh a complicated design (~80,000 faces) for a microchannel heat sink, as pictured, and I would appreciate some advice. I have tried a range of different mesh controls...

08 October 2019 5,610 7 View

How to get higher concentration of linearised plasmid?

I am Trying to use Infusion cloning to prepare a mutant construct. I have been told by a student that used it before that I need 20ng/ul of linearised plasmid to proceed with the following...

04 April 2019 646 10 View

Who are leading theorists today on memory ?

Specifically memory when writing memoir.

03 April 2018 3,172 2 View

I wish to inactivate mammalian source phospholipase activity, can anyone offer insight or references to published work?

I'm looking to mitigate in vitro phospholipid degradation over extended periods of time.

20 February 2018 8,740 3 View

Are likelihood-based approaches to statistical modelling REALLY removing the arbitrary problem of 95% significance?

It strikes me that likelihood-based approaches are prone to the similar dichotomous decisions as traditional hypothesis testing. E.g. In the environmental sciences we now seem to accept that...

14 October 2017 1,264 8 View

Can you silane treat polyurethane plastic to make it more hydrophobic?

06 August 2017 3,205 5 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Are there any candidates for an Einstein's bare cosmological constant?

It is commonly accepted that General Relativity has its own 'bare' cosmological constant that contributes together with the cosmological constant resulting from vacuum energy density for an...

23 February 2021 5,448 8 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

How Nanotechnology helps to develop various products in Agrometeorology?

Nanotechnology (NT) is one of the advanced material science with which the development in materials and improvement in electronics. NT has made it possible to miniaturizing the things to fit in...

21 January 2021 2,305 6 View

Can you give me an example of a faulty or egregious environmental claim that is NOT related to climate change?

I am doing lesson plans and am looking for examples of environmental claims that do not stand up to close scrutiny. There are more than enough examples of this related to climate change...so no...

18 January 2021 4,661 7 View

How to use Cantera's equilibrium program to calculate adiabatic temperature of dust flame?

To calulate the adiabatic flame temperature using Cantera, we need to provide a .cti file that stores all thermodynamic information needed. We can simply convert a Chemkin mechanism file to .cti...

13 January 2021 7,072 2 View

Are the mice expected to develop obvious airway disease after HDM? Will they have signs of respiratory distress at any time during the study?

I am developing model of asthma in mice using HDM. I was asked this question in order to submit the a protocol for developing a disease in animals. Searching in the literature, I can not find any...

30 December 2020 6,455 3 View

How can the amount of accumulated dust be estimated?

Hi Kind helpers, For my industrial design thesis, I'm working on a concentrated solar energy (>CPV) construction, to be integrated in urban areas/buildings (glass roofs, with integrated...

28 December 2020 9,180 8 View

How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Weibull fading matlab codes ?

hello I tried to looking for Weibull fading channel matlab codes and i found just the following formula: h = lambda.*(-log(rand(nRx(jj),N)).^(1/beta)) my question is this codes express the...

09 December 2020 6,575 1 View