Any recommendation on SaCas9 antibody?

Hi, does anyone know a good SaCas9 antibody for IHC or immunofluorescence on mouse brain slides? We gave a try with the Epigenetek monoclonal antibody (A-9001) without antigen retrieval (1:500)...

29 December 2020 4,368 5 View

Why GFP fluorescence disappear in nude mice?

ESCC cell line KYSE180-GFP (GFP lentivirus transfected into KYSE180) were subcutaneously injected to nude mice. GFP fluorescence is properly detected under in vivo imaging system 7 days after the...

04 May 2020 8,068 4 View

Why can not my protein run into the gel during the BN PAGE?

29 March 2016 7,230 6 View

Is Histcite still available and could someone help me with the retrieval issue?

15 December 2014 2,284 9 View

How much tissue is needed for DARPP-32 western blot from rat medial prefrontal cortex?

Anyone tried DARPP-32(Thr34 and Thr75) on mPFC tissue? Does frozen tissue from fresh decap work for these assays?

23 January 2013 386 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,366 3 View

Which is the best wavelength to study aerosol optical depth(AOD) related to climate reseach,which criteria must be adopted in choosing the wavelengh?

We are doing a study on impact of aerosol on precipitation. Which satellite data and which wavelength will be the best. or can some one suggest a criteria to be adopted to choose an instrument...

12 February 2021 6,324 3 View

Ligands with 3,4,5-trihydroxyphenyl group?

Hi In the design of a bidentate ligand with imine group, is it okay to have a 3,4,5-trihydroxyphenyl group in the structure? In other words, does the 3,4,5-trihydroxyphenyl group compete with...

08 February 2021 4,977 7 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

For Carbon-emission free steelmaking by electrolysis with nonoxidizable electrode in EAF, what would be electrode material?

While CCS can be employed along with a conventional Carbon-efficient EAF steelmaking process (especially from iron ore) or even Hydrogen or hydrocarbon can bee passed through central electrode,...

30 January 2021 3,378 1 View

Is it possible to prepare clay minerals by mixing pure oxides of required elements? What would be theoretical difficulties with it?

Practically this is not at all necessary. But theoretically, would hydrous/hydrothermal synthesis be enough (like mix 1 mole alumina gel and 4 mole silica gel and gently heat) or would it require...

26 January 2021 6,403 3 View

How Nanotechnology helps to develop various products in Agrometeorology?

Nanotechnology (NT) is one of the advanced material science with which the development in materials and improvement in electronics. NT has made it possible to miniaturizing the things to fit in...

21 January 2021 2,305 6 View

Can you give me an example of a faulty or egregious environmental claim that is NOT related to climate change?

I am doing lesson plans and am looking for examples of environmental claims that do not stand up to close scrutiny. There are more than enough examples of this related to climate change...so no...

18 January 2021 4,661 7 View

Seperate of organic and inorganic phosphorus in waste water?

Is it possible to separate organic and inorganic phosphorus in wastewater with a filter?

14 January 2021 952 3 View