We are using 7,12-dimethylbenz[a] anthracene (DMBA) and Croton oil combination to induce breast cancer in Swiss albino mice. Is there any other method to do so effectively?
I think that it will be useful to use orthotopic injection of Ehrlich ascites carcinoma , details pf it provided at this poster http://www.bbcs.net.br/uploads/aulas/3084171fbd52bae6ab7bf6b99f3e9daa.pdf
If you are looking for a personalized protocol to help with your research question then I would suggest you use ProtocolNow (www.protocolnow.co). Its a platform that can identify qualified experts from anywhere in the world to help design protocols for scientific peers.
The protocols are designed specifically for the experiment needed, include detailed steps as well as explanations to help understand the purpose of each step, best reagents to use (based on scientific experience) and recommendations for the interpretation and analysis of the data.
Use 4T1 mouse breast cancer cell line. Well established for breast cancer . 10 lakh cells in sterile pbs a booster dose in tits of mice. Mammary gland. Wait for 2-3 days. If not developed again 5 lakh cells additional dose. Am sure it will work.