What are the different techniques to transfer a gene from Gateway compatible cloning vector to any retroviral/lentiviral vector for transfection ?

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What is the physical meaning for the following equivalent circuit?

Can anyone help me to find out the Rs = Solution resistantce and Rct= Charge transfer resisistance of the following equivalent circuit. specifically, which R should I take as Rct. I got this...

12 May 2024 4,358 2 View

Minimize the sum of squared errors between the experimental and predicted data in order to estimate two optimum parameters by using matlab?

In my research work, I want to do the vehicle survival fraction; survival rates were calculated using the log-logistic survival function. In equation two unknown parameters a, and b are obtained...

30 March 2024 2,074 0 View

Can a particle be its own antiparticle?

Quantum Entanglement is a phenomeno inwich

03 February 2024 3,685 4 View

How to generate topology for Graphene molecule from .gro file using GROMACS program?

Greetings, everybody. I have generated a graphene sheet using the VMD program and saved the resulting file in the .gro format. Now I would like to use the GROMACS program to model the interaction...

17 December 2023 8,093 1 View

Can you help me with state-space modelling of Virtual Synchronous Machine based STATCOM?

I am currently working on the state-space modelling of Virtual Synchronous Machines (VSM) based STATCOM (Static Synchronous Compensator). The focus of this work is to develop a comprehensive...

11 December 2023 2,275 0 View

In Hyperspectral Classification, computed accuracy are very close to existing state-of-the-art methods. Then why statistical tests are not applied?

Hyperspectral Imaging, Hyperspectral Classification, Statistical Test

08 November 2023 4,900 2 View

What would be the optimal way to convert a 2D .sdf chemical library into .pdbqt for AutoDock Vina docking?

I am attempting to convert several .sdf ligands (2D) into .pdbqt files using the Open Babel program for molecular docking. I have followed the steps below: Add Hydrogens Generate 3D...

04 October 2023 1,484 3 View

Assertion failed error in gromacs during protein ligand complex tutorial?

I am trying to perform simulation of my protein-ligand complex system following Gromacs Protein-Ligand complex tutorial , so during the NVT equilibration using the command " gmx grompp -f nvt.mdp...

14 September 2023 9,378 2 View

How to determine the solvation box size and volume after performing production run in GROMACS?

Solvation Box

08 August 2023 3,685 0 View

Regarding new refrence sequence of wheat?

Is there any database for the KEGG pathway, Gene enrichment analysis, network analysis, etc. in wheat that accepts the gene ID of RefSeq 2.0 (recent version)?

26 July 2023 140 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View