Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Why gel electrophoresis is taking too much time to run ?

I am working with TBE buffer and prepared 1% agarose gel. Previously it was taking around one hour to run but now two and half hours. I have increased the voltage from 90 to 110 but this also helped.

08 December 2020 7,416 5 View

What are the recent research topics in field of Soft Robotics based on AI and ML?

I am working in the field of practical robotics since last five years and got acquainted with AI and ML recently. I know Python and learning AI and ML using python from different sources. I want...

24 October 2020 793 6 View

Can Solar Energy take part in fighting against COVID-19?

Can "Solar-UV-cellular-damage" be an effective method to disinfect water bodies against COVID-19 in countries with a hot climate?

10 May 2020 2,556 12 View

While performing energy minimization step in GROMACS I am getteing following error. Can anyone help with this minimization problem?

gmx mdrun -v -deffnm em Back Off! I just backed up em.log to ./em.log. Running on 1 node with total 8 cores, 16 logical cores Hardware detected: CPU info: Vendor: GenuineIntel Brand:...

11 March 2020 2,902 1 View

What is iodine number in terms of activated carbon? What are the effect of iodine number on activate carbon porosity ?

11 March 2020 5,239 3 View

How to convert Netcdf file to ASCII data file?

I want to convert NetCDF file containing MODIS LAI data into ASCII file but not getting enough help from google search. if anybody is familiar with this, please give some inputs.. I am using R and...

18 February 2020 6,100 5 View

HI, Can I engineer any immortal human T cell line with the murine TCR of Ovalbumin ? Just like OT1 mice T cell have the same receptor ?

I am planning to develop a Human T cell line having the potential to kill OVA expressing tumor lines. Mostly we have to take out Tcells from OT1 mice which is expensive in overall cost. If I...

25 December 2019 3,417 2 View

What is the best suitable High Voltage amplifier to excite PZT ceramic C-91 patch (fuji ceramics ltd.)?

I need some information regarding a suggestion for piezo linear amplifier/High voltage amplifier suitable to operate at a peak voltage of 500V/mm for piezo ceramic patches (Rectangular C-91)....

27 November 2019 8,466 8 View

How we encapsulate vitamin-D with chitosan nanoparticles and how much conc. of ethanol we use to dissolve vitamin-D ?

We are trying to encapsulate vitamin-D in chitosan. Kindly suggest me the solubility of vitamin-D in ethanol and the simplest method to encapsulate vitamin-D. Thanks with regards

05 November 2019 9,341 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View