Vikas Ranvir

5 Questions 11 Answers 0 Followers

Questions related from Vikas Ranvir

Is it necessary to put a linker between fusion proteins?

I want to fuse my gene of interest with EGFP and mCherry for localization studies. I am wondering if immdiate presence of mCherry or EGFT after my protein of interest would cause any steric...

21 March 2019 9,229 2 View

How long the mammalian cells can be stored in methanol at -20 for fixation during immunocytochemistry/immunofluorescence?

During the fixation step of immunocytochemistry/immunofluorescence, some protocols say that cells can be stored at -20 for a week, while some protocols advice to keep it for 10 minutes to...

15 January 2019 4,688 3 View

What are the different techniques to transfer a gene from Gateway compatible cloning vector to any retroviral/lentiviral vector for transfection ?

I am trying to transfer a gene from pDONR 221 vector into pBABEpuro-gateway vector using Gateway cloning but facing problems. Is there any other technique by which I can transfer the gene from...

07 December 2018 5,369 2 View

What is the best method to dissolve Syringaldazine (SGZ) for performing laccase assay?

I am trying to dissolve SGZ to be used as substrate for Laccase activity assay. I tried dissolving SGZ in absolute ethanol but it is not dissolving completely, more ever it is forming suspension...

11 May 2017 3,172 6 View

Which is the best linker used to improve solubility and stability of fusion proteins ?

I am working on cloning & expression of fusion proteins. There are poly Glycine linkers to improve flexibility between fused proteins. Are there any other linkers available which will serve to...

22 September 2016 1,637 2 View