Vikas Ranvir

8 Questions 15 Answers 0 Followers

Questions related from Vikas Ranvir

Is it necessary to put a linker between fusion proteins?

I want to fuse my gene of interest with EGFP and mCherry for localization studies. I am wondering if immdiate presence of mCherry or EGFT after my protein of interest would cause any steric...

21 March 2019 9,300 2 View

How long the mammalian cells can be stored in methanol at -20 for fixation during immunocytochemistry/immunofluorescence?

During the fixation step of immunocytochemistry/immunofluorescence, some protocols say that cells can be stored at -20 for a week, while some protocols advice to keep it for 10 minutes to...

15 January 2019 4,735 3 View

What are the different techniques to transfer a gene from Gateway compatible cloning vector to any retroviral/lentiviral vector for transfection ?

I am trying to transfer a gene from pDONR 221 vector into pBABEpuro-gateway vector using Gateway cloning but facing problems. Is there any other technique by which I can transfer the gene from...

07 December 2018 5,426 2 View

Why genetic code selected only 20 amino acids as proteogenic amino acids?

There are more than 150 naturally occurring amino acids, then why protein synthesizing machinery adds only those 20 amino acids in protein sequence ?

11 March 2018 9,787 3 View

Why/how does the atomic number of an element increase by +1 in case of β-decay?

During β-decay, an element ejects one electron. Then how does its atomic number increase by +1 even if it is loosing 1 electron?

13 January 2018 8,213 4 View

While designing fusion proteins, is it okay if I keep start codon in the sequence of second protein ?

While designing the fusion of two proteins, are there any chances that the start codon of the second protein carry out translation independently, leading to formation of only second protein...

02 November 2017 5,000 0 View

What is the best method to dissolve Syringaldazine (SGZ) for performing laccase assay?

I am trying to dissolve SGZ to be used as substrate for Laccase activity assay. I tried dissolving SGZ in absolute ethanol but it is not dissolving completely, more ever it is forming suspension...

11 May 2017 3,237 6 View

Which is the best linker used to improve solubility and stability of fusion proteins ?

I am working on cloning & expression of fusion proteins. There are poly Glycine linkers to improve flexibility between fused proteins. Are there any other linkers available which will serve to...

22 September 2016 1,685 2 View