I am using human neutrophils as a model for an experiment. Each time I need resting neutrophils, so before asking for blood from a blood bank, I need to know whether can I revive resting neutrophils from this blood. I have tried cryopreservation of human neutrophils and I got 80% death during revival. Will I get the same kind of problem during neutrophil isolation from whole blood from a blood bank?

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