What are the differences in relative qPCR quantification and absolute RNA quantification
In relative quantification you quantify the expression of your gene as a fold difference from a endogenous control called housekeeping gene (HKG). The HKG expression must not suffer any alteration due to your biological question so you can quantify if your gene of interest (GOI) is upregulated or downregulated.
In absolute quantification you quantify the exact quantity by extrapolating a calibration curve made with 10fold dilutions of a known concentration template. Some people use a linearized vector cloned with the GOI, PCR amplified GOI, synthetic nucleotide, etc.
...the the answer is correct, however digital pcr has overcame all the techniques aforementioned in absolute quantification and rare allele detection. you will never have an absolute quantifuication, I mean exact count of the number of templates, because in real time there is always a certain variability (although small) due to technical and operational proficiency
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