Which is the best silane for making a borosilicate glass surface hydrophobic permanently? and what is the protocol?

Regarding my needs I am looking for a glass coating agent that makes the glass hydrophobic permanently

09 February 2021 3,924 5 View

Why the difference between degrees of nonclassicality of the input states is required for nonzero entanglement in the output state of a beam splitter?

By considering two single-mode Gaussian states at the input of a beam splitter, it is seen that the entanglement of the output two-mode Gaussian state depends on the difference between the degrees...

22 January 2021 3,490 3 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View

Which kind of GPC solvent can be used to dissolve both lipid and salt?

I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).

14 January 2021 3,805 2 View

Hi Everyone. I want to conduct DEMATEL analysis for my survey. Everything is in line except the MAXIMUM MEAN DE-ENTROPY (MMDE) ALGORITHM. Need help ?

I can't find any manual or procedure on how to conduct the MMDE process. It looks like most of the literature has copy-pasted the stuff without any method of how to do to it. Any practical book,...

07 January 2021 1,804 2 View

The saturation point does not reach in my ITC. Does this indicate that the interaction is weak or there is no interaction?

21 December 2020 9,407 8 View

Does anyone have experience in infecting suspension cells (like THP-1 or K562 etc) by retrovirus?

I am trying to make stably expressing cell line of my gene of interest in Suspension cell line using retrovirus but i don't have recommended protocol. I have searched literature but mostly they...

14 December 2020 8,274 3 View

Hello I have a question about solar cells (perovskite solar cells) modeling in COMSOL?

I have done all optical simulations in RF module, now how can i find generation rate of electrons and holes, secondly how can i use these generation rate in semiconductor module to find further...

11 December 2020 6,652 3 View

Can a mathematical model be added to a paper on bean genetic and diseases manuscript?

Can a mathematical model be added to a paper on bean genetic and diseases manuscript?

10 December 2020 2,300 14 View

Straight chain or Ring peptide for inhibiting a homo-dimer of a 20 KDa secretory protein?

I am designing a small peptide to inhibit a homo-dimer of a small secretory protein (about 20 KDa). I need suggestions on, which option would be better? a straight-chain peptide or a ring-shaped...

03 December 2020 4,145 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View