What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How to compare two group with small sample size (n=3)?

I am working on some physiological traits of plant to which its expensive to raise the sample size beyond n=3. so kindly suggest some test which is applicable to test such group(2)

04 February 2021 461 11 View

Spatial thinking and geographical reasoning: what are their conceptual differences and interrelations in teaching geography?

I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.

17 January 2021 1,123 3 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

How or from where can I get energy load profiles for the household, tertiary and industrial sectors specifically for EU27 countries?

Dear Researchers, I am looking for time series or a function to generate yearly, monthly or daily load profiles for the sectors such as household, tertiary and industrial. These load profile must...

11 January 2021 2,307 2 View

Cross country comparison with matched pairs design?

Hi, I'm doing a cross-country comparison. My data for each country has a match pair design i.e. each company within a country is match with a control firm from that country. The data is...

07 January 2021 4,983 6 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

20 December 2020 1,222 4 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

What is role of chronopharmacology or circadian rhythms in metabolic diseases including obesity, diabetes and cardiovascular diseases?

Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...

18 January 2021 9,045 3 View

How can I do the bang's blinding index and james blinding index?

Hi! I would like to know with which statistical software the bang blinding index and the James blinding index and how to do it. Thanks

09 December 2020 1,363 2 View

Using Circwave or JTK_cycle for qPCR data?

Hello everyone, My experiment is to assess the expression levels in cultured cells after the clock has been reset, or in mouse liver by qPCR. I have multiple timepoints and have performed...

21 November 2020 802 2 View

What is the value of dynamic viscosity and bulk viscosity of extracellular matrix (ECM), nucleus, and cytoplasm of cell?

Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...

19 October 2020 8,729 1 View

How transfected DNA is transported into the nucleus, to get stable integration?

What kind of mechanisms are involved in the integration and transportation of transfected DNA/Gene to the nucleus?

08 October 2020 2,329 2 View

Best Cabinets for circadian rhythm studies in mice?

For those working with mouse models of circadian rhythm disorders, or using changes in the light-dark cycle to induce circadian disruptions, what is (are) the best options for cabinets during the...

07 October 2020 1,319 1 View

How Blind and Visually Impaired can Navigate in indoor areas?

Dear all, I am thinking to develop solutions for blind people to navigate and positioning themselves in indoor areas such as malls, hospitals, etc. I am thinking about the tool or device that can...

18 September 2020 8,671 7 View

What is the best way to detect ATF4 activation? And ATF6?

To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you

17 September 2020 2,973 1 View