Hi
Check the document below.
http://www.gelifesciences.co.jp/catalog/pdf/18116379.pdf
You can used any buffer that have next characteristics:
- the ph and composition is within the range of application of the column (ph mainly)
-your protein is completely soluble in the buffer
-able to generate a good baseline at the wavelenght employed.
I would suggest phosphate buffers or tris hcl buffer at phisiological pH
ok, thank you all : )
i will be use phosphate buffer
so, which can i dissolve the BSA, in water or phosphate buffer?
I would use the phosphate buffer used for elution. After solving the protein in this buffer youcan either centrifuge or filtrate the sample to remove any insoluble compound, which could block the column. If you dissolve your protein sample in any other buffer, it might happen that once the protein is in contact with elution buffer it precipitates becasue the solubility is different.
Also remember that BSA is an equilibrium between monomer and dimer. You might want to choose a protein that performs nicer (only one quaternary state).
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