How to overcome the tension or frustration with others who are less organized or careless than you are?

Working with others usually good and build a massive amount of experiences, but sometimes there are some drawbacks of it. Careless, less organized, randomness, and commitment are some important...

30 October 2020 2,520 8 View

Transient heat transfer through 2 walls and to air?

Dear All I am trying to simulate a problem that simplified as shown in the attached file. It has two different walls one inside the other and surrounded by air. I want to simulate the problem in...

14 October 2020 1,002 19 View

How many volume for media that we need to isolate exosomes from media?

Because i isolate 5 ml of culture media without serum and i got 1.2 ng miRNA . And i need 70 ng minimum that we can do PCR

28 September 2020 5,887 4 View

Is there any research points link Statistics and Big Data analytics that can be good points for research in PhD ?

Statistics and Big Data Analytics

02 August 2020 2,830 6 View

Which companies provide Molecular imprinted polymers ( MIPs) ?

19 July 2020 8,876 9 View

What are the differences between Renewable Energy and Energy Efficiency Technologies?

I found that energy efficiency deals with combustion efficiency, using biomass fuels and stuff like that. Can we link between wind energy and energy efficiency by some means?

07 June 2019 2,892 6 View

What are the best mesh quality criterion for inspection element quality?

I know about mesh density, skewness, wrap angles, and aspect ratio. Per literature, aspect ratio is a quick tool to define quality of mesh. Is there a rule of thumb for acceptable values of...

07 February 2019 7,145 4 View

Is Fmincon solver suitable for nonlinear ratio (I.e. NLP/NLP)?

I use fmincon to minimise weight to stiffness ratio. This ratio is Non linear. I tested this solver by using different initial points Xo. However, results are changeable and dependent on Xo. I am...

01 February 2019 3,069 3 View

What are the main considerations in injection mold designing and calculations?

I'd like to know the main parameters that should be considered in designing of injection mold. Also, I need to know a source for standardized calculations.

10 February 2018 6,412 3 View

Is there a relation between pore size and agarose gel concentration?

17 April 2017 6,408 8 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,220 3 View

What is the better software for approximation of 3D surface with equations?

I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?

21 February 2021 3,444 7 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View