7 Questions 10 Answers 0 Followers
Questions related from Kiyohiro Takahasi
Hi, I'm trying to start protein NMR again. When I was doing protein NMR 10 years ago, I used NMRpipe for data processing. But I think NMRpipe is not user friendly. Please teach me any other...
30 January 2022 6,586 4 View
Hi My protein of interest is only expressed in E. Coli insoluble fraction. So, I tried to extract them adding 6 M guanidine to the insoluble pellet. By rotating the mixture of pellet and solution...
12 March 2021 1,532 3 View
Hi I have expressed and purified 16 kDa protein with N-terminus His-tag and C-terminus polyLys tag. I measured the 1D NMR spectrum and found this protein has folded structure and also found the...
06 September 2018 8,423 5 View
Hi Now I'm trying to express and purify human protein using E. Coli. The protein size is 14 kDa and pI is 8.2. I made two expression vectors carrying the target protein using pGex6p1 for...
26 August 2017 7,519 4 View
I'm planning to make N-terminal split GFP fused recombinant proteins using E. Coli. So I want to make the expression vector with multi cloning site for target protein at the 3' of the split GPFs....
03 February 2016 636 3 View
Hi I am going to try E.Coli Rosetta 2 (DE3) pLyss strain for protein expression. I have read some papers and found pLys strain does not need sonication for lysing because of Lysozyme expression....
02 December 2015 2,976 3 View
I'm trying to purify membrane protein that is expressed in E. Coli. I have read several papers about lysing E. Coli Cells to purify target membrane protein. And found most of the researchers use...
11 March 2015 8,119 4 View
