My group mates and I were tasked to prepare "perfect and permanent" slides for one of our classes. Sadly, it did not work out the way we wanted it to. As a result, we created some over-stained and some under-stained samples and slides that had bubbles.

When placed under a microscope, we found some black structures that were inside the cells. We want to determine what they are, since there aren't that many studies about the Ficus nota. It may be a component of the cell, a contaminant of some sort, or dirt from the slide.

We used safranin as the stain. Through research, we have not found any sources that say anything about safranin staining creating black components, other than the cell wall, given the right procedures. The sample preparation procedure involved the leaf undergoing killing and fixing solution (made up of ethanol, formalin, and acetic acid), bleaching solution (bleach, NaOH, and water), various ethanol and xylol series of increasing concentrations, and staining by safranin.

It would be much appreciated if one of you could help us with our project. If there are any further questions about the study, feel free to ask. But bear with me due to my limited knowledge on the topic, as I am still a student. Thank you!

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