Hi Martiijn sir,

Denitification can be worked out indirectly as follows:

Analyse initial soil Total N

Analyse Total nitrogen in soil after leaching with water in a column

Analyse leached nitrates

If it needs to be tested after addition of fert., accordingly after the treatment leaving 15 days the same may be done.

Initial Total N + added N if any = x value

Total N after leaching = Y value

Nitrate N in leachate = Z value

Denitrification value =  X - (Y+Z)  if the soil pH is acidic

Denitrification will be still less if the soil pH is alkaline where ammonia volatilization is also accounted.

Thanks

Thank you for the information, it's much appreciated!

Martijn