Due to the low polarity of your target isolates which are steroids, I suggest you start with 100% hexane as your mobile phase on the column chromatography packed with silica gel stationary phase of your preferable mesh size. You can then follow up with the combination of hexane:ethyl acetate 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70......till 100% ethyl acetate. You then conduct TLC for the collections to see the profiles. Pool the collections that has similar TLC profiles and proceed with further purification following similar process till you have a single homogenous spot on TLC using two or three solvent systems. Good luck