Enzymes and interleukins are commonly used for assessments, as well as in vivo animal studies. Are there another models of assessments?
Hi Salmon. We have recently characterised a set of markers etc that can be used for this purpose. We propose that by exposing microglia (or macrophage) to LPS or IL-4 plus your extract of choice to identify how the novel compound modulates reactivity. We have used PCR and Luminex for cytokines/chemokines and various cell surface markers. In addition the effects of conditioned media from the treated microglia on neuronal viability. It is aslo possible to use functional assessment of inflammatory cell activities, such as phagocytosis or migration. These are also valuable and tell you about the way changes in expression alters actual functioning of cells that is important in inflammation. Importantly, no one marker will be able to tell you about immunomodulatory potential and effects over tim are also very critical to assess.
Article Characterization of phenotype markers and neuronotoxic poten...
Many thanks Bobbi, but I guess other pathways of inflammation were not explored in your studies, such as the role played by anti-oxidants, enzymes and other pro-inflammatory mediators. Any chance you may forward me your full research article (pdf). Your input is much appreciated.
Hi Salmon ! Very interesting question. We are actually working with INRA colleagues and with Cohiro on this purpose. We have developed human cell models to investigate the inflammatory/anti-inflammatory effects of plant extracts. If you are interested in collaborative work do not hesitate to contact me.
Sincerely,
Jean-Louis
NB: Cohiro has a great experience in plant extract testing either on cell models or on mice inflammation models that they are now running.
Dear all,
I also find this question interesting. I developed a perfusion system with which liquids and buffers (e.g. with inflammation provoking substances) can be exchanged in a second and without interrupting or perturbing the perfusive flow. However, biologists doesn't seem not to be interested, though its really powerful annd allows for very clean measurements. Please contact my for any collaboration or co-development. I would be delighted about it.
Cheers Claus (www.biophysical-tools.de & www.ipfdd.de)
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