Research focused on alternative and cheaper sources for NSAIDs and DMARDs.
The in vitro anti-inflammatory activity of any compound can be assessed in LPS induced RAW 264.7 cells (murine macrophages). Markers to assess include TNF-a, IL-1, IL-6. You can also estimate ROS and nitrite levels.
You can try TNF-a, IL-1b, IL-6, etc. However, apart from the biomarkers, a improvement on pathology is very important to make sure your compounds are active.
The choice of the cell lines is very important to study the anti-inflammatory compound. I agree with Aiken. Better go with In vivo data for changes in Joint cytokine levels and histopathology
The in vitro anti-inflammatory activity of any compound can be assessed in LPS induced RAW 264.7 cells (murine macrophages). Markers to assess include TNF-a, IL-1, IL-6. You can also estimate ROS and nitrite levels.
IL-12 is a good biomarker too, details given in this link
http://www.copewithcytokines.de/cope.cgi?key=IL12
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology