Is there always a relationship between amount of disulfide bonds and rigidity of a protein? what about amount of alpha helix and rigidity?

Lysozyme is consisted of 4 disulfide bridges while the number of disulfide bridges for BSA is 17. Nevertheless, lysozyme is known to be a more rigid protein. What are the other factors affecting...

11 December 2019 1,231 4 View

What is the general protocol for ThT assay?

We are working on the fibrillation of lysozyme. Given the acidic condition (pH 2) of the protein solution (glycine buffer) for fibrillation, what should be buffer and its pH for ThT stock...

05 June 2019 9,954 4 View

How to identify the structure of polymer-NP conjugate?

We have conjugated a polymer with nano particles. The CHNS analysis revealed that a number of around 25 nano particles were conjugated with each polymer. Now, I want to know if it is a core-shell...

09 October 2018 4,879 7 View

Is there any article about refolding process of proteins with quaternary structure?

05 June 2016 6,189 6 View

What is the chemical and physical situations for the formation of amide and disulfide bonds?

Please introduce me the general protocol for in vitro formation of amide and disulfide bonds, respectively.

03 April 2015 3,567 3 View

Can anybody help me in the selection of sec gel type?

I want to determine the percentage of unfolded, native and aggregated form of my protein in a sample. As some researchers in this site have suggested, I want to use sec chromatography. The...

04 May 2014 3,016 8 View

Can anyone help with combination of SDS-PAGE and SEC?

It's better to describe my question with an example : Suppose that we have a protein solution and SEC (size exclusion chromatography) indicates that it has 10% of aggregated form and SDS-PAGE...

03 April 2014 3,579 4 View

Is there any specific protocol for the preparation of high performance size exclusion chromatography?

I am going to use sephadex g-50 in my research

01 February 2014 8,682 4 View

What is the simplest way to make aggregates from a solution of unfolded proteins?

I need a sample that contains aggregates for testing my chromatography process.

01 February 2014 5,721 7 View

A useful method for evaluating the percentage of native protein in a solution?

I want to evaluate the percentage of native Interferon beta-1b after refolding process.

07 August 2013 4,938 6 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View