Reza Fattah

11 Questions 10 Answers 0 Followers

Questions related from Reza Fattah

Is there always a relationship between amount of disulfide bonds and rigidity of a protein? what about amount of alpha helix and rigidity?

Lysozyme is consisted of 4 disulfide bridges while the number of disulfide bridges for BSA is 17. Nevertheless, lysozyme is known to be a more rigid protein. What are the other factors affecting...

12 December 2019 1,303 4 View

What is the general protocol for ThT assay?

We are working on the fibrillation of lysozyme. Given the acidic condition (pH 2) of the protein solution (glycine buffer) for fibrillation, what should be buffer and its pH for ThT stock...

06 June 2019 10,019 4 View

How to identify the structure of polymer-NP conjugate?

We have conjugated a polymer with nano particles. The CHNS analysis revealed that a number of around 25 nano particles were conjugated with each polymer. Now, I want to know if it is a core-shell...

10 October 2018 4,931 7 View

What are the best methods to find the numbers of PEG molecules around each PEG-coated nanoparticle?

We have synthesized a PEG-coated nanoparticle (Fe3O4@PEG). Now we want to estimate the average number of PEG molecules around each nanoparticle (or maybe the average molecular weight of each...

06 June 2018 2,339 3 View

Is there any article about refolding process of proteins with quaternary structure?

I am looking for a review article that explains experimental approaches for refolding of proteins with quaternary structure or an article about a protein that has quaternary structure.

06 June 2016 6,240 6 View

What is the chemical and physical situations for the formation of amide and disulfide bonds?

Please introduce me the general protocol for in vitro formation of amide and disulfide bonds, respectively.

04 April 2015 3,621 3 View

Can anybody help me in the selection of sec gel type?

I want to determine the percentage of unfolded, native and aggregated form of my protein in a sample. As some researchers in this site have suggested, I want to use sec chromatography. The...

05 May 2014 3,088 8 View

Can anyone help with combination of SDS-PAGE and SEC?

It's better to describe my question with an example : Suppose that we have a protein solution and SEC (size exclusion chromatography) indicates that it has 10% of aggregated form and SDS-PAGE...

04 April 2014 3,673 4 View

What is the simplest way to make aggregates from a solution of unfolded proteins?

I need a sample that contains aggregates for testing my chromatography process.

02 February 2014 5,781 7 View

Is there any specific protocol for the preparation of high performance size exclusion chromatography?

I am going to use sephadex g-50 in my research

02 February 2014 8,784 4 View

A useful method for evaluating the percentage of native protein in a solution?

I want to evaluate the percentage of native Interferon beta-1b after refolding process.

08 August 2013 5,002 6 View