Please introduce me the general protocol for in vitro formation of amide and disulfide bonds, respectively.
The question is unclear. Are you asking about the formation of amide or disulfide in cell culture?
Please find this excellent review article on "How ribosomes make peptide bonds."
http://www.sciencedirect.com/science/article/pii/S0968000406003240
I am asking for the formation protocol of each of them separately.
I am looking for the general in vitro procedure of their formation; not any particular application.
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