What are the best method for determination of oxidative stress in rat's uterus?

More Ali Saeed Al-Chalabi's questions See All

PCR primers stopped working, any reason why?

The first few qPCR runs the primers worked and I was getting signals in my samples/positive controls. However, now when I run another qPCR with the same primers I get no signal. I've tried using a...

17 October 2022 746 5 View

Why is my LightCycler taking longer to run qPCRs?

I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is...

17 October 2022 9,072 3 View

Please , I need (XRD) data sheet standard (JCPDS manual). for ( hydroxyapatite, multi-walled carbon nanotubes, High-density polyethylene ) materials?

XRD data sheet standard JCPDS manual

01 February 2020 7,466 3 View

Where to find EEG dataset for anesthesia?

I am looking for EEG dataset for anesthesia, please where can I find such dataset?

10 November 2019 6,786 3 View

In clustering, How are graphs of KNN built?

I’ve found out several clustering algorithms such as chameleon or even Spectral Clustering. its work to converting data to a weighted. KNN graph based on the distance between points. I'd like to...

09 October 2019 6,802 4 View

What are the current challenges to be considered to solve in Scientific Workflow scheduling in cloud computing?

Scientific Workflow scheduling in cloud computing and I need suggestions on the gaps in this domain. The gaps that would need to be directions for future works?

08 September 2019 4,527 2 View

Is Baron And Kenny 1986 Mediation Approach Out Of Date?

HI RG colleagues, some statistics' comments believe that the Baron And Kenny 1986 mediation method out of date. they suggested new effective approaches in term of testing the mediating variable.

07 August 2019 4,191 15 View

Is the google scholar h-index a good representative for the strength of the researches? what are the drawbacks of the index ?

The advantages of this index are evident. The h-index can obviously be applied to any set of papers. Since the h-index cannot exceed the umber of underlying papers, many authors...

06 July 2019 6,316 3 View

Is it necessary to follow the instructions of the journal in regard to the number of words?

The number required by the journal for the publication of articles does not exceed six thousand words, while the total words of article nearly 8000.. Should I follow the journal instructions in...

06 July 2019 7,211 33 View

Is it critical to move on from MATLAB to Python in ANN field?

Although I realize the success made using Python for different aspects of ANN and deep neural networks. However, I am a MATLAB user. Is it worth it to learn Python for that purpose? or is MATLAB...

04 May 2019 8,500 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Tobias M. Ntuli

The malondialdehyde assay is the standard procedure (Leprince et al., 1993)! Our lab has used the magnesium chloride procedure (Ntuli et al., 1997, 2011, 2013).

Ali Saeed Al-Chalabi

Thank you very much for your advice , please can you send to me the procedure that you mentioned ?

Please try the references indicated!