I've got a Roche LightCycler that I use to run my qPCRs. It used to take 1h 37 min to run a qPCR but now it take 1h 45 min using the same exact settings. Could the problem be that the machine is taking longer to reach certain temperatures? Does that mean it needs servicing?
Thanks!
Are you certain that none of the settings have been changed? It could mean that it's taking a bit longer to change temperatures. Might contact a sales representative to get their opinion if they is expected or unusual.
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Thanks, Dan
The room temperature could make some differences, usually in summer with AC on they take a little bit longer, but if the room Ta is not drastically modified, a call to the maintenance service could be a must. Also, the reading lamp have to be regularly checked if you hadn't so.
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