I would like to know what are the best markers to study Th1, Th2, Th17 and Treg T cells in mouse by FACS. I want determine the proportions of these populations and their activation profile.
Thank you.
CD4+ T cells are key regulators of the adaptive immune system and can be divided into T helper (Th) cells and regulatory T (Treg) cells. During an immune response Th cells mature from a naive state into one of several effector subtypes that exhibit distinct functions. The transcriptional mechanisms that underlie the specific functional identity of CD4+ T cells are not fully understood.
Results
To assist investigations into the transcriptional identity and regulatory processes of these cells we performed mRNA-sequencing on three murine T helper subtypes (Th1, Th2 and Th17) as well as on splenic Treg cells and induced Treg (iTreg) cells. Our integrated analysis of this dataset revealed the gene expression changes associated with these related but distinct cellular identities. Each cell subtype differentially expresses a wealth of ‘subtype upregulated’ genes, some of which are well known whilst others promise new insights into signalling processes and transcriptional regulation. We show that hundreds of genes are regulated purely by alternative splicing to extend our knowledge of the role of post-transcriptional regulation in cell differentiation.
Conclusions
This CD4+ transcriptome atlas provides a valuable resource for the study of CD4+ T cell populations. To facilitate its use by others, we have made the data available in an easily accessible online resource at www.th-express.org.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384382/
@Mehdi Rostami Rad
What about the IHC not gene makers for Th and Treg cells
I chose Tbet for Th1
RORgamma for Th17
ST2 for Th2
Th cells can be identified based on (i) cell surface markers, (ii) cytokines signature and (iii) transcriptional regulators.
For eg. Th1: (i) CXCR3, WSX-1, IFN-gamma R2, IL-18R, CCR5, (ii) IFN-gamma, IL-2, TNF-alpha, TNF-beta (iii) STAT1, STAT4 and T-bet. Th2: (i) CXCR4, CCR3, CCR4, CCR8, ST2/IL-1R4, IRF4 (ii) IL-4, IL-5, IL-13, (iii) STAT5, STAT6, and GATA-3.
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