Jack Chang

7 Questions 8 Answers 0 Followers

Questions related from Jack Chang

Would you represent a Glucose Tolerance Test with glucose levels as percent of time 0?

Hi, I have performed a glucose tolerance test (GTT) on mutant vs WT mice and I have no statistical difference in glucose levels (mg/dL) during the test between the mutant and WT mice, and no...

06 November 2017 6,606 3 View

For what reason(s) do auto-antibodies anti-insulin could be elevated in the blood of mice?

Hi, I have a mutant mouse model in which there are increased auto-antibodies against insulin in the blood. Does someone know why these autoantibodies could be elevated in mouse blood? Thank you.

05 October 2017 9,198 0 View

Does inflammation decrease or increase gluconeogenesis ?

Hi, I would like to know if inflammation stimulates or inhibits gluconeogenesis. Inflammation is known to trigger insulin resistance, which should lead to an increased gluconeogenesis since...

22 August 2017 5,642 3 View

In which tissue insulin resistance is the most critical to develop type 2 diabetes?

Hi, I would like to know if insulin resistance in one tissue in particular contributes more to type 2 diabetes development than in other tissues. Thank you.

21 August 2017 440 2 View

What are the best markers to study Th1, Th2, Th17 and Treg CD4+ T cells ?

I would like to know what are the best markers to study Th1, Th2, Th17 and Treg T cells in mouse by FACS. I want determine the proportions of these populations and their activation profile. Thank you.

12 July 2017 4,842 4 View

What is the best way to count the cells of the stromal vascular fraction (SVF) of mouse adipose tissue?

I have difficulties to count the adipose tissue SVF cells using a Neubauer cell because there are very few cells and a lot of debris. What is the more efficient way to count these cells? Thank you.

07 July 2017 4,801 5 View

How a change in gluconeogenesis genes expression levels can affect the gluconeogenesis pathway flux and glucose production?

Hi, I have a decrease of 1/3 to 1/2 in the gene expression of gluconeogenesis enzymes (Glucose-6-phosphatase, Fructose-1,6-bisphosphatase, Phosphoenolpyruvate carboxykinase and Pyruvate...

06 July 2017 8,145 5 View