I am planning on doing ADCC and cytotoxicity assays. Which methods I can use that are sensitive and with minimum background. I do not want to do 51Cr release assay as it involves radioactive Cr.
I am preparing a radiolabeled product using a radiolabeled substrate. I have the cpm values for different dilutions of both my known substrate and unknown product ? How can one determine the...
27 July 2024 3,604 1 View
MY RESEARCH TOPIC IS THE IMPACTS OF KIRI DAM ON SWEET POTATO CULTIVATION FOR FARMERS OF THE UPSTREAM COMMUNITIES, ADAMAWA STATE, NIGERIA. I NEED RELATED AND LATEST ARTICLES (2020 TO DATE) TO...
22 June 2024 4,117 1 View
Can Sensory marketing affects customers satisfaction in Hotels?
12 May 2024 3,308 3 View
Can you please provide CIF file , Input SCF file
10 December 2023 986 2 View
I have an article describing a procedure but no citation in it.
21 October 2023 343 1 View
I need to do a BET test on the Amberlite XAD-4 adsorbent and the operator needs the following parameters before he can proceed. 1. Atom diameter 2.Polarizability 3. Magnetic susceptibility 4....
11 October 2023 4,798 1 View
Someone is trying to create a new account using my email. An activation request was forwarded to me to activate the email account severally earlier, and even today by 08:06am.
30 July 2023 9,835 0 View
What could be the possible reason for not getting proper band of ladder while performing PAGE
07 June 2023 7,641 1 View
I have knocked out genes which will most likely produce male sterile plants. It is a part of my master's thesis. However, I find myself somewhat perplexed regarding the number of tests that need...
07 June 2023 1,260 0 View
I am using the Laviron equation and putting the value of alpha 0.4, scan rate 0.05V/sec, delta E value is 0.1 V. I have put all the values but still not getting the answer. Could anyone please...
30 August 2022 237 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View