There are many undiscovered natural products out there in nature. How do scientists spot a specific genus of plant for their target molecules? Do they follow the linage of the plant and keep on extracting and see what they get?
When studying a certain protein, purifying the protein fused with His tag can obtain a high concentration of protein, but precipitation is prone to occur. We found that whether this protein is...
27 March 2023 8,828 0 View
I am studying the binding of a bacterial protein without a crystal structure to a small molecule. Through predicted structure and molecular docking, I have found several amino acid sites with...
06 February 2023 3,201 3 View
Dear colleague, I am testing on formulation of cationic lipid nanoparticles. The lipid compostiin is DOTAP: DSPC: Cholesterol: PEG2000 DSPE=50: 10: 39: 1. The buffers of 50 mM sodium acetate at...
11 December 2021 2,800 1 View
Dear Colleagues, To design a final stage process development for lipid nanoparticle formation using microfluidics, what aspects should be taken into account? Thank you for your...
09 August 2020 880 3 View
I have defined the UDF with DEFINE_EXECUTE_AT_END and DEFINE_SDOF_PROPERTIES. I want to update the load of the SDOF with every time step, but I can't do it. Which Macros should I use? Thank you
16 April 2019 706 3 View
I am working on ultrasound image quality that's why i need some vital link.
26 January 2019 935 5 View
I am trying to use heart beat as a developmental index to study the effect of environmental contaminants. I will be using MS-222 (Tricaine methanesulfonate) to anesthetize the larvae.
30 October 2018 3,566 6 View
Dear all, I am currently doing a project that is integrating the GIS and ANN together for watershed management. However I am stucked in how to inputting the files into the neural network. Please...
19 February 2018 7,987 5 View
Hi everybody, Please could you tell me the best filtering cutoff (specially for MAF and missing data) I should apply to my SNPs called from GBS data before doing population structure analysis in...
18 June 2017 3,815 5 View
I'm looking for any medical conditions that trypsin concentration significantly increases in the disease state. Any information on the concentration of trypsin in normal environment versus disease...
02 February 2016 9,981 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Are increasing the costs of disasters in the affected countries.
01 August 2024 1,794 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...
01 August 2024 8,754 2 View