Did somebody tried to use the attR1 and attR2 sites without the chloranphenicol resistance gene i.e. AttR1-ccdB-AttR2?

I am trying to design a vector as compact as posible to test DNA transcriptional enhancer and/or repressor activity, I already have a compact bacbone (pICOz vector), minimal promoter, translation...

22 August 2020 1,083 1 View

Could the E. coli J53 strain conjugate a plasmid that it received?

E. coli J53 is always used as a recipient strain, but could it conjugate later?

05 March 2020 4,093 8 View

How can I remove PEI coating?

12 September 2017 2,558 6 View

Does anyone know how to use Sentinel 1 data in agriculure to estimate biomass?

31 October 2016 7,395 3 View

What is your opinion about this delta k?

11 July 2016 8,644 8 View

Is there relationship between asphalt roughness and SAR backscattering coefficients? Could somebody suggest a reference (articles, textbooks etc.)?

SAR backscattering coefficients asphalt roughness roads quality evaluation

06 July 2016 9,781 3 View

Can I insert two genes (from different organisms) which encode the same type of protein, in a single vector ?

10 June 2016 3,664 3 View

How can one determine the plasmid size (in Dalton) according to kb or bp gel markers?

I would like to determine plasmid sizes in Dalton but I do not have reference strains with standard plasmid to make the inference. By using Lambda PFG marker, for instance, is it possible to...

06 March 2015 9,852 2 View

How can we treat externalities of media as "market fails", without giving pretext to censorship?

It is notorious that broadcasting is one of the means where free speech and freedom of opinion are made effective, and also it is notorious that it produces positive and negative externalities....

12 January 2013 1,186 1 View

Does the agenda setting concept fit social networks?

The concept of agenda setting is fundamental in studying mass media. There is a great question if we can say that social network has amplified the universe of discussion beyond all that is treated...

03 January 2013 1,830 7 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

ECG and children with autism ?

Does anyone have experience with collecting ECG data on children with autism? How was your experience? I'm interested in the tolerance levels for kids to have wires attached to their chests. What...

02 February 2021 5,850 3 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

Anyone who used chelating agents during DNA extraction?

I am struggling to extract DNA from mine tailings even after enrichment due to suspected metal ions degrading DNA or inhibiting PCR reactions. I have used the following DNA extraction kits Zymo...

26 January 2021 4,542 2 View

List any intelligent frequently ask questions among poeple regarding special need education and people with special need?

i need any question that is related to the special need education or the people special need (exceptional children)

25 January 2021 7,851 6 View