I am trying to design a vector as compact as posible to test DNA transcriptional enhancer and/or repressor activity, I already have a compact bacbone (pICOz vector), minimal promoter, translation enhancer, reporter and terminator and I would like to transform my reporter construct to the gateway system to test starr-seq libraries. As I have to transform the empty vector as control and in addition I co-transform with other plasmids to test transformation efficiency it is very difficult to transform my plant cells with big plasmids, thats why I am trying to do it as compact as posible. My question is if somebody tried to use a the ccdB suicidal gene alone to negatively select recombinant clones. It normaly comes with chloranphenicol resistance. I can save more than 1.2 kb.
I may not be understanding your proposal, but I think the answer to your question is that would not work. ccdB is a negative selection, but you still need a positive selection in the system to distinguish from non-transformants.
If you really want to minimize the size, there were some tiny plasmids built years ago that used a tRNA suppressor (supF or suIII) as a selectable marker and a host strain carrying a resistance gene with a nonsense codon. The tRNA gene was I believe less than 200nt. Check the PiVX system from some decades ago.
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