Discrepancy protein levels and receptor expression?

I measured the expression of a protein that is a receptor in a panel of cell lines. When I measured the protein levels by Western blot the protein levels were about the same in all cell lines ,...

09 September 2020 6,671 3 View

Can I insert sequence (200bp-800bp) in pUC57 plasmid for PCR and qPCR applications??

I have to PCR assays. I want to prepare positive controls with DNA synthetic. But, I'm not sure use pUC57 plasmid. My PCR product are between 200 -800 bp. What other considerations should I have?

02 February 2020 4,585 2 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

27 February 2018 1,151 2 View

How to calculate the surface coverage of two adjacent but no overlapping cellular structures?

Dear all, I am staining two adjacent cellular structures solids with fluorescent antibodies (Alexa-488 and 555). I want to calculate the coverage of the small solid respect to the big one. In...

12 February 2018 2,606 3 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

01 February 2018 4,079 2 View

No title

26 February 2017 4,268 6 View

What's the meaning of anti-disolvent?

suspension of particles in a non-aqueous anti-solvent

01 February 2017 4,805 0 View

Where can I find mean pairwise relatedness coefficients for MULE DEER (Odocoileus hemionus)?

We did a study on a free-ranging population of this species, in which we tested whether spatial associations (2 adult deer within 25 m of each other) varied with several factors such as season,...

20 April 2016 165 4 View

What statistical technique I use and how I use it to analyze a comparison between a native strain and control strain for the solubilization ofcopper?

evaluated the bioleaching of a native strain compared with a control strain, have variables (cell concentration,% solubilization of copper, time) and the taking of the data is that starting from a...

13 April 2016 8,557 1 View

How to measure codesign performance?

03 April 2016 7,502 2 View

Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Another hyaluronic acid content analysis in cell culture?

Is hyaluronic acid content analysis by carbazole assay applicable to cell experiments? I'm looking for hyaluronic content analysis not use ELISA kit.

17 November 2020 6,990 2 View

How to culture of keratinocytes from human skin punch biopsy?

Hi, I have been having problems culturing keratinocytes from 4mm human skin punch biopsies. When I recieve the biopsy I first was the samples in sterile PBS, then I remove the subcutaneous fat. I...

24 April 2020 2,696 2 View

Collagen gelation in organotypic culture of fibroblast and primary keratinocytes?

Dear all, I am culturing human primary keratinocytes and fibroblast together to make an organotypic model of skin. I already had the following collagen solution in my lab so I used...

10 March 2020 4,269 3 View

How to isolate keratinocytes from fibroblasts in a co-culture using medium?

Currently I'm culturing primary human fibroblasts and keratinocytes from a skin punch in medium (DMEM with 20% FBS and 1% P/S). I would like to culture these cells separately, but I was wondering...

01 March 2020 9,425 3 View

How to perform a lipid peroxidation assay?

Hello everyone, I would like to measure MDA levels on keratinocytes using a lipid peroxidation assay, I have searched for protocols in published literature but aren't clear enough since I have...

18 February 2020 4,273 3 View

How to perform a lipid peroxidation assay?

Hello everyone, I would like to measure MDA levels on keratinocytes using a lipid peroxidation assay, I have searched for protocols in published literature but aren't clear enough since I have...

01 February 2020 3,935 3 View

Which cell lines used as alternative to immortalized human keratinocyte cell lines (HaCaT) to asses antipsoriatic activity ?

suggest alternative cell lines for HaCaT cell lines for the assessment of antipsoriatic activity

31 December 2019 1,558 3 View

What could be the Reason of Keratinocytes are growing in 75Flasks but not in same treated 96 Wells?

We work with OKG4 gingival Keratinocytes between Passage 13-18 and cultivating them on Collagen 4 in 75cm Flasks and implement them with 2x10*4 cells on 96 Well with 0,6cm growth area and 5x10*5...

18 November 2019 7,322 2 View

Where i can get HaCaT cells (as an immortalized human keratinocyte cell line) ?

for checking antipsoriatic activity

10 November 2019 745 1 View