In general first you need to have pure cultures of the isolates. second, probe differen tculture media to see in which of them  the isolate produce itssexual/asexual reproductive structures. Then compare its structures with those registered in fungi compemdium. Thus you know the class and genus  to which belong the isolate. Later based on morphometric features of the reproductive structures  you can  identify  thespecies.

Hi Sohel: This is some useful papers:

http://www.caes.uga.edu/content/dam/caes-website/departments/plant-pathology/extension-pdfs/PDL-fungi-key.pdf

http://www.ascofrance.fr/uploads/forum_file/Humber-RA-Chapter-V-Entomofungi-identification-0001.PDF

https://books.google.com/books?id=gq8h2Q6KckEC&pg=PA388&lpg=PA388&dq=identify+fungal+key+hyphomycetes&source=bl&ots=d769G0jGPG&sig=VU2VbD_jKMsAMJJ0fdp94hm6eCY&hl=en&sa=X&ved=0ahUKEwi7nN-58uXSAhVP4mMKHRybABEQ6AEIUjAJ#v=onepage&q=identify%20fungal%20key%20hyphomycetes&f=false

https://www.ars.usda.gov/ARSUserFiles/80620520/APSwkshoprev.pdf

http://conference.ifas.ufl.edu/npdn/Docs/Workshop%20Materials/NPDN%20Microfungi%20Workshop%20Manual.pdf

http://www.britmycolsoc.org.uk/files/5513/3026/1433/Keys_to_Fungi_on_Dung-category%202%20%20Keys%20useful%20but%20not%20current..pdf

Thanks for your answers i will refer the literature given above

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Hi Sohel

Some references maybe help you

http://www.springer.com/cda/content/document/cda_downloaddocument/9789048197347-c2.pdf?SGWID=0-0-45-1120158-p174024884

2

http://revistamexicanademicologia.org/wp-content/uploads/2013/06/RMM-Tr269-version-paginada-19-27.pdf

3

http://bookshop.europa.eu/fr/fungal-identification-techniques-biotechnology-1992-94--pbCGNA16510/downloads/CG-NA-16-510-EN-C/CGNA16510ENC_001.pdf;pgid=y8dIS7GUWMdSR0EAlMEUUsWb0000Ykkn1avC?FileName=CGNA16510ENC_001.pdf&SKU=CGNA16510ENC_PDF&CatalogueNumber=CG-NA-16-510-EN-C

4

https://www.fungaldiversity.org/fdp/sfdp/FD_3_29-38.pdf

5

http://cdn.intechopen.com/pdfs/34844/intech-molecular_tools_for_detection_of_plant_pathogenic_fungi_and_fungicide_resistance.pdf

6

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3359816/

7

http://biblioteka.ihar.edu.pl/show_pdf.php?src=pdf/04/99b74c43.pdf&name=Marcinkowska%20Joanna%20Z..pdf

First of all, you should use morphological identification methods. Basically, use some media inoculate petri dishes for a week. Then put  a fungal piece on the slides examine under a microscope then use with  identification key books. If you can identificate for example genus level you should use PCR methods with species specific primers or send any company for sequences.

Thanks for your answers

you are welcome