I found white pink colonies on my media plates. May I know, are they bacteria or yeast ? If it is bacteria , is it possible to be E.coli or Staphylococcus spp.? If it is yeast, is it possible to be Candida spp?
You have not mentioned the name of medium on which pink color colony grew.
You please do the wet mount preparation in Narayan Stain and do microscopic examination under high power.If you find circular/ oval cells with or without budding, it is yeast. Rhodotorula is yeast that gives brown color on Sabouraud dextrose agar and Pal sunflower seed medium. We have developed Narayan stain and Pal sunflower seed for the study of fungi.
It is edible agar medium. Is it Possible **for the pink yeast( Rhodotorula spp) to grow on edible agar medium? My project is on identification of bacterial contaminants on media plates after exposing the media plates in different area in Seed Production lab for 1 hour , then the media plates were collected and incubated them for 5 days. The experiment was carried to check sterility of the lab.
This may be fungus (Rhodotorula spp) grows on culture plates as a smooth-pink colonies consisting of ovoidal yeast, about 10 microns in diameter. Anyway do microscopic examination .
Thank you for the information Prof. Yes, I do have the same thought. Yeast are fungi, generally has larger colonies, with a flattened edge and appear glossy and glistening on plate. I believe it is (Rhodotorula spp). Yes I will try to do microscopic examination to enhance the contrast.
Though we are working on on fungi pathogenic to humans and animals since 1973, we never used edible agar medium. Pal sunflower seed medium, which we developed in 1980, has been used to study the mycoflora of hospitals, abattoir houses, poultry farms, vegetable markets etc.
It looks like Rhodotorula sp. You have to conduct microscopic and biochemical tests to confirm it. And yes it can grow on Edible agar medium. Hope it helps.
These pink colonies are typically coliform bacteria in the family Enterobacteriaceae, including, but not limited to, the genera Escherichia, Klebsiella, Enterobacter, Hafnia and Citrobacter. The presence of coliform bacteria is commonly used as an indicator of unsanitary food and water.