I am working on a research that involves working with strains known to be over-producers of various classes of efflux pumps. I have seen that most of the research involving efflux pump over-expression are done using strains like SA-1199B, XU212, RN4220, E. coli K12 AG100 etcetera.
However, I would like to know if there are any ATCC or other commercially available strains of Staphylococcus aureus, Escherichia coli or Pseudomonas aeruginosa which show good/strong efflux pump activity.
Bacteria can become resistant to antibiotics via three mechanisms: antibiotic inactivation, target modification, and alteration of intracellular antibiotic concentration. The latter mechanism can occur by either decreasing permeability to an antibiotic or increasing the activities of a variety of efflux pumps. While permeability is a significant barrier to antibiotics in gram-negative bacteria due to the presence of an outer membrane, it is an unlikely mechanism of resistance for gram-positive bacteria, since they lack an outer membrane. Efflux of antibiotics is a clinically significant general resistance mechanism for bacteria,
ATCC provides top-quality microbial strains needed to maintain outstanding Quality Control programs. Commercial firms specify ATCC strains as controls for rapid identification, minimum inhibitory concentration of antibiotics and antibiotic susceptibility panels. Most professional organizations that draft standards require that cultures not be used beyond five passages from the original ATCC strain. Trust ATCC for your laboratory quality control needs.
the following link may help you
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546547/
Hi Shadid! I hope it's going well!! Did you eventually find other strains with strong efflux activity?
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