Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What could cause these "frowning" bands seen in electrophoresis (SDS-PAGE)?

As part of my western blotting procedure, I ran my SDS-PAGE electrophoresis. Usually, I load in 2 lanes, 1 ladder (spectra broad range protein ladder) and my sample (sample lysate + Laemmli...

30 October 2019 6,607 16 View

Cell preparation for Folch method?

Hello, I'm going to extract lipid from HepG2 cell by Folch method. Usually, for tissue, we put 100 mg of tissue into glass vial, then add 5 mL of chloroform:methanol:distilled water (2:2:1 v/v)....

06 January 2019 3,154 2 View

Is there any substitute for Apiezon W wax?

I'm planning to transfer GaAs film to other substrate (e.g. Si) using Apiezon W wax. My GaAs sample has "GaAs/AlAs/GaAs substrate " structure so I have to use wax (or epoxy) that can resist HF or...

12 April 2015 1,192 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

IC50 value from this graph?

01 January 2021 6,848 4 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

I am performing enzyme inhibition assay and the substrate is fluorogenic & fluorescence was measured. Now I want to calculate Vo and Vi. How can I ??

These are the per minute fluorescence observed. Please guide me how can I determine Vi, Vo and IC50 ? By slope determination or any other method? Control: 28.11 40.30 40.78 37.61...

11 December 2020 1,037 4 View

How to determine non-cytotoxic drug concentration using MTT assay?

Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...

31 October 2020 2,012 3 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

How do I find the IC50 and best drug treatment time for anticancer drug?

I want to find the IC50 of a drug on a cancer cell line. I'm not sure how long the treatment time should be for in vitro experiment. Is there an assay/protocol I can follow to determine the IC50...

15 October 2020 8,678 6 View